Artificial cultivation method for wild agaricus campestris and application

A reed mushroom and artificial technology, applied in the field of artificial cultivation of wild reed mushrooms to obtain fruiting bodies, can solve the problems of extinction, reduction and aggravation of wild reed mushroom yield and quality

Active Publication Date: 2017-09-29
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to the destruction of Qilihai’s ecological environment, water sources have gradually dried up, wetlands have been degraded, more and more wetlands have been filled for agriculture or construction, and the area of ​​reeds has decreased. It needs to grow under the influence of common environmental factors such as specific temperature, humidity, light and soil. The combination of the above environmental and human factors will eventually lead to a decrease in the yield and quality of wild reed mushrooms in Qilihai Wetland, Tianjin. According to local folk reports in July 2016 Environmentalist Lu Shaosheng made a comprehensive follow-up survey and statistics. Wild reed mushrooms in this area are hard to find and are very likely to face the danger of extinction. After consulting the data, there has been no relevant report on the success of artificial domestication, which will undoubtedly further exacerbate the situation of their extinction.

Method used

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  • Artificial cultivation method for wild agaricus campestris and application
  • Artificial cultivation method for wild agaricus campestris and application
  • Artificial cultivation method for wild agaricus campestris and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1 strain separation

[0058] The present invention adopts the tissue separation method to obtain strains, selects some fruiting bodies of wild mushrooms with normal color and no damage by diseases and insect pests, removes surface debris, and wipes them with 75% alcohol. Cut the fruiting bodies longitudinally into two, take mung bean-sized tissue pieces at the junction of the stipe and cap, quickly insert the tissue pieces into a PDA plate with tweezers, and culture them in the dark for 10-15 days in a constant temperature incubator at 29°C.

Embodiment 2

[0059] Embodiment 2 Morphology, molecular biology identification

[0060] (1) Observation of colony morphology and structure: Observe its growth on the plate every other week, and take pictures to record the color and growth of mycelium.

[0061] (2) Insert culture: insert culture, after the mycelium climbs to the cover glass, take it out and make a temporary mount, and observe the structure, type, branch, etc. of the mycelium under an optical microscope.

[0062] (3) Observation of the morphological characteristics of the fruiting bodies: records of cap size, color, shape, gills, stipe, etc.

[0063] 3 ITS identification process

[0064] (1) Cultivation of experimental materials

[0065] Take wild reed mushroom colonies that grow vigorously on the plate, transfer them to the prepared liquid medium in a clean bench aseptically, and culture them in the dark at 29°C. Mycelium can grow in about 15 days.

[0066] (2) Genomic DNA extraction reagents

[0067] ①Pick an appropriat...

Embodiment 3

[0084] Example 3 Screening of suitable temperature for mycelium growth

[0085] The PDA comprehensive medium was cultured at 5 different temperatures of 14°C, 19°C, 24°C, 29°C, and 34°C. Each temperature treatment was repeated 5 times, the diameter of mycelium was measured with a vernier caliper, and the average value was taken, and the growth of mycelium, color of mycelium and characteristics of colonies were recorded.

[0086] 1. Preparation of plate culture medium

[0087] Wash the petri dishes and sterilize under high pressure at 121°C and 0.10-0.12Mpa for 30 minutes before use. Turn on the ultra-clean table ultraviolet lamp, sterilize for 20 minutes, pour the melted PDA culture medium into a petri dish (volume is 20ml), cover and cool.

[0088] 2. Inoculation operation

[0089] Wait for the PDA medium to solidify before inoculating. Turn on the ultraviolet lamp for sterilization, and turn off the ultraviolet lamp after 20 minutes. Aseptically pick a 5mm square bacter...

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Abstract

The invention relates to an artificial cultivation method for wild agaricus campestris and application. The artificial cultivation method comprises the following steps: separation of mother strain pure culture, establishment of mother strain culture conditions, optimization of a culture formula, establishment of conditions for transformation from mycelium to a primordium, establishment of sporocarp culture conditions, artificial cultivation to obtain the sporocarp of the agaricus campestris, and feature description by the method in the morphology and the molecular biology. By the method, artificial cultivation of the wild agaricus campestris can be carried out. The artificial cultivation method has the advantages of low production cost, simplicity in operation, large quantity of primordia, capability of obtaining the sporocarp and the like. The growing period of the sporocarp of the cultivated agaricus campestris is about 60 days. The artificial cultivation method provides strong support on protection of diversity of species of a wetlands ecosystem, saving of resources of endangered wild mushroom germplasm, development of intensive study in the later period, and implementation of reasonable and ordered development and utilization of the wild agaricus campestris. The artificial cultivation method for wild agaricus campestris has quite high protection, study, development and promotion value, and is remarkable in economic benefit and ecological benefit.

Description

technical field [0001] The invention belongs to the technical field of edible fungus processing, and relates to an artificial cultivation method and application of wild reed mushrooms, more precisely, a method for artificially cultivating wild reed mushrooms to obtain fruiting bodies. Background technique [0002] Tianjin Qilihai Wetland is a national-level wetland nature reserve, located in Ninghe District, northeast of Tianjin, with a total area of ​​about 95 square kilometers. The wetland is rich in a kind of wild mushroom, which is called reed mushroom by the local people. It has a unique taste and is nutritious. Rich, delicious and unique, loved by the locals, because of its scarcity and high price, this mushroom occurs in the thick humus after the summer weather is sultry and rainy. In recent years, due to the destruction of Qilihai’s ecological environment, water sources have gradually dried up, wetlands have been degraded, more and more wetlands have been filled for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 刘西周郭成金吕绍生李兆江
Owner TIANJIN NORMAL UNIVERSITY
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