Avian adenovirus strain, inactivated vaccine and preparation method

Abstract

The invention provides a fowl adenovirus strain, an inactivated vaccine and a preparation method. The fowl adenovirus strain is named a fowl adenovirus TJ strain with a preservation number of CCTCC (China Center For Type Culture Collection) NO:V201632; the adenovirus strain is of race C in a fowl adenovirus I subgroup, the serotype is 4 type, viral nucleic acid is double stranded DNA (Deoxyribonucleic Acid), and no envelope is found; the diameter of virus particles is 70 to 90nm, and the virus particles are of icosahedral symmetrical structures; the inactivated vaccine is prepared by performing cell inoculation, venom harvesting, inactivating and emulsifying on a fowl adenovirus; a vaccine strain is a prevalent strain, and is good in immunogenicity and high in multiplication capacity; the prepared vaccine has the advantages of high safety, good stability, quick production of immune response, high virus challenge protecting rate, and the like.

Description

Technical field [0001] The invention relates to a virus strain and an inactivated vaccine, in particular to an avian adenovirus strain, an inactivated vaccine and a preparation method, and belongs to the technical field of biological engineering. Background technique [0002] Chicken Inclusion Body Hepatitis-Hydroperioadium Syndrome (IBH-HPS) is a recently prevalent, acute and highly contagious chicken infectious disease, and its pathogen is Fowl adenovirus (FADV) , In the virus classification, it is Adenoviridae, avian adenovirus of the I subgroup of the avian adenovirus genus. The virus mainly infects broiler chickens, 817 hybrid broiler chickens, and hemp chickens aged 1-3 weeks. It is also more common in broiler breeders and laying hens. Among them, broiler chickens aged 3-7 weeks are more affected. [0003] The disease has spread nationwide in China, mainly in Shandong, Henan, Anhui, Liaoning, Jilin, Hebei, Jiangsu and other places. The disease is mainly transmitted vertical...

AI Technical Summary

Problems solved by technology

[0004] In terms of diagnosis, the current diagnosis is mainly based on clinical symptoms, histopathological changes, PCR amplification, and virus isolation; in terms of disease prevention and control, although there have been research reports on the use of chicken embryos and poultry-derived cells to culture avian adenoviruses, However, the serotypes of most of the reported strains are inconsistent with the current market strains. Therefore, the current market urgently needs to develop a poultry gland with a serotype consistent with the current market strains, high titer, and good immunogenicity. virus vaccine