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A core-shell nanofiber scaffold and its method for constructing tissue engineering materials with melanocytes

A nanofiber, core-shell type technology, applied in the field of biomedicine, can solve the problem that human melanocytes cannot be cultured, and achieve the effect of good mechanical properties, good shape, and maintaining activity.

Active Publication Date: 2018-08-24
HANGZHOU THIRD HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The core-shell nanofiber scaffold patent disclosed above is used for the sustained release of drugs and cannot be used for the cultivation of human melanocytes

Method used

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  • A core-shell nanofiber scaffold and its method for constructing tissue engineering materials with melanocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1. Preparation of chitosan-polyethylene oxide core-shell nanofiber scaffold material

[0048] A. chitosan is dissolved with trifluoroacetic acid / dichloromethane weight ratio 3:1 mixed solvent, is made into the shell solution that concentration is 5%;

[0049] B. Dissolve polyethylene oxide, polyethylene glycol methacrylate, and polyethylene glycol dimethacrylate in water at a weight ratio of 80:20:1 to form a 5% nuclear layer solution , add 1% photoinitiator 1-[4-(2-hydroxyethoxy) phenyl]-2-hydroxyl-2 methyl-1-propane-1-one before spinning;

[0050] C. Electrospinning is carried out under the conditions of voltage of 15KV, core-shell propulsion rate of 0.5ml / h, receiving distance of 15cm, temperature of 30ºC, and ultraviolet light irradiation;

[0051] D. Bake the core-shell nanofiber support under vacuum at 50°C for 12 hours;

[0052] E. At 20ºC, the core-shell nanofiber scaffold was cross-linked with sodium sulfate for 2 hours, and after the cross-linking...

Embodiment 2

[0056] Example 2. Preparation of chitosan-polyvinylpyrrolidone core-shell nanofiber scaffold material

[0057] A. Dissolve chitosan with acetic acid / water (weight ratio 1:1) mixed solvent to make a chitosan solution with a concentration of 2%;

[0058] B. Dissolve polyvinylpyrrolidone, hydroxyethyl methacrylate, and N,N'-methylenebisacrylamide in water at a weight ratio of 70:30:3 to form a 9% nuclear layer solution. Add 3% photoinitiator phenyl bis(2,4,6-trimethylbenzoyl) phosphine oxide before spinning;

[0059] C. Electrospinning is carried out at a voltage of 20KV, a shell-layer propulsion rate of 0.5ml / h, a core-layer propulsion rate of 1ml / h, a receiving distance of 18cm, a temperature of 20ºC, and ultraviolet light;

[0060] D. The chitosan / polyethylene oxide-polyvinylpyrrolidone core-shell nanofiber scaffold was baked under vacuum at 25°C for 24 hours;

[0061] E. At 20ºC, the chitosan-polyvinylpyrrolidone core-shell nanofiber scaffold was cross-linked with genipin f...

Embodiment 3

[0065] Example 3. Preparation of gelatin-polyvinyl alcohol core-shell nanofiber scaffold material

[0066] A. Dissolve the gelatin with trifluoroethanol / water (mass ratio 8:2) to make a 10% gelatin solution;

[0067] B. Dissolve polyvinyl alcohol, methacrylic acid, and ethylene glycol dimethacrylate in acetic acid / water (mass ratio 1:1) at a weight ratio of 60:40:0.4, and the concentration is 3%. The core layer solution, add 0.2% photoinitiator 2-hydroxyl-1-[4-(hydroxyl) phenyl]-2-methyl-1-propanone before spinning;

[0068] C. Electrospinning is carried out at a voltage of 25KV, a shell layer propulsion rate of 1ml / h, a core layer propulsion rate of 0.5ml / h, a receiving distance of 20cm, a temperature of 40ºC, and ultraviolet light;

[0069] D. Bake the gelatin-polyethylene glycol nanofiber scaffold under vacuum at 25°C for 24 hours;

[0070]E. At 50ºC, the gelatin-polyvinyl alcohol core-shell nanofiber scaffold was cross-linked with glutaraldehyde for 12 hours, and after t...

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Abstract

The invention discloses a core-shell type nano fiber bracket and a method for constructing a tissue engineering material by the core-shell type nano fiber bracket and melanocytes. A core-shell type nano fiber is prepared through electrostatic spinning; a shell layer is a biocompatible material and a core layer is in-situ cross-linked polymer hydrogel. The tissue engineering material is constructed by using the nano fiber bracket and the melanocytes or the nano fiber bracket, mechanocytes and keratinocytes, and can be used for treating depigmentation (such as leucoderma). The core-shell type nano fiber bracket disclosed by the invention has good biocompatibility and biodegradability, and can be used for loading medicines and realizing controllable releasing of the medicines; a tissue engineering bracket constructed by the core-shell type nano fiber bracket can be used for effectively supporting the growth, multiplication and transplanting of the melanocytes and co-culture cells.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a core-shell nanofiber scaffold with drug sustained release function and a method for constructing tissue engineering materials with melanocytes. Background of the invention [0002] Vitiligo is a common multiple depigmentation skin disease. The clinical manifestations are mainly localized or localized white patches with clear boundaries and no symptoms. Histology and immunocytochemistry show that the epidermal melanocytes in the lesions disappear. . Vitiligo has an incidence rate of 0.5-2% in the population. Due to the lack of melanin in the leukoplakia, it is easy to be burned by the sun and even cause cancer. At the same time, because it affects the appearance of the human body, it often causes serious psychological obstacles to patients and reduces their quality of life. Traditional treatment methods mainly include drug therapy and light therapy. But drug therapy and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/16A61L27/20A61L27/22A61L27/24A61L27/38A61L27/50A61L27/60D01F8/02D01F8/10
CPCA61L27/16A61L27/18A61L27/20A61L27/222A61L27/225A61L27/24A61L27/38A61L27/3804A61L27/3813A61L27/50A61L27/60A61L2400/18D01F8/02D01F8/10C08L5/08C08L29/14C08L33/08C08L29/04C08L33/10C08L71/02
Inventor 许爱娥王文俊吴非凡尉晓冬
Owner HANGZHOU THIRD HOSPITAL
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