Application of alpine yarrow herb polysaccharide as vaccine adjuvant
A technology of vaccine adjuvant and polysaccharide, which is applied in the field of application of polysaccharides in vaccine adjuvants, can solve the problems such as undiscovered polysaccharides of polysaccharides, and achieve the effects of large-scale production, wide sources and high yield
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Embodiment 1
[0033] Example 1: Animal experiment of different doses of Artemisia polysaccharides on mouse immune model antigen ovalbumin (OVA)
Embodiment 1
[0034] The preparation method of present embodiment one artemisia polysaccharide is:
[0035] Step 1: Degrease fresh or dried Artemisia argyi powder with petroleum ether, add distilled water for ultrasonication, add absolute ethanol to stir and precipitate the filtrate after concentration, centrifuge the precipitate, and dry it in vacuum to obtain a crude product of Artemisia argyi crude polysaccharide;
[0036] Step 2: Use Sevage reagent (chloroform: n-butanol ratio: 4:1) to remove protein, centrifuge, combine supernatant, repeat the above operation several times, collect supernatant, add ethanol, let stand overnight, and precipitate Centrifuge and dry in vacuum to obtain the crude polysaccharide of Artemisia argyi.
Embodiment approach
[0038] Experimental material: Artemisia argyi polysaccharide mother liquor configuration: 0.1g of Artemisia argyi crude polysaccharide was dissolved in 10ml of normal saline to prepare a mother liquor of 10mg / ml. Prepare OVA mother solution: dissolve 0.01g OVA in 10ml of normal saline, and prepare a 1mg / ml mother solution.
[0039] Animal immunization: 6-8 week-old ICR mice were randomly divided into 8 groups, 6 mice in each group, and the experimental groups are shown in Table 1.
[0040] Table 1 Immune grouping of mice
[0041]
[0042] Each group of vaccines was dissolved in normal saline, and each mouse was injected with 100 microliters, subcutaneously on day 0, and re-injected at an interval of 14 days after the first injection, a total of two injections.
[0043] In order to detect the level of humoral immunity in mice, blood was collected after subcutaneous injection, the serum of mice was separated, and IgG and IgG were detected by indirect ELISA respectively. 1 a...
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