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Superparamagnetism nanoparticles, preparation method therefor and applications

A superparamagnetic and nanoparticle technology, applied in the nano field, can solve the problems of small saturation magnetization, large particle size of contrast agent, poor water solubility and physiological stability, etc., to increase stability, improve stability, and have superior magnetic response. Effect

Active Publication Date: 2015-09-16
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the method of synthesizing the superparamagnetic contrast agent that has been approved for marketing and is being clinically tested is mainly obtained by the co-precipitation method, but this type of contrast agent has large particle size, low crystallinity, low saturation magnetization, poor MRI imaging effect, and surface coating. The thickness of the coating is uneven; while another type of superparamagnetic iron oxide nanoparticles synthesized by high temperature pyrolysis, although the particle size is smaller and more uniform, the magnetic properties of iron oxide are superior, but its water solubility and physiological stability are serious defects. It hinders its application to in vivo imaging, so surface remodification is required to improve water solubility and physiological stability and its targeting

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  • Superparamagnetism nanoparticles, preparation method therefor and applications

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Embodiment 1

[0069] In this embodiment, the superparamagnetic iron oxide nanoparticle contrast agent is prepared by the following method, which specifically includes the following steps:

[0070] (1) Take 3g of ferric chloride and dissolve it in a mixed solvent of 40mL of distilled water and 40mL of ethanol to form solution a (concentration of solution a is 37.5mg / mL);

[0071] (2) Weigh 17g of sodium oleate and disperse it in 80mL of n-hexane to form solution b (that is, the mass fraction of oleate is 23.5wt%);

[0072] (3) After mixing solutions a and b, heat to 60°C, stir and reflux for 4h. After washing with water, dry in vacuum to obtain solid d;

[0073] (4) Dissolve 1 g of solid d in 20 mL of octadecene, add 0.5 mL of oleic acid, stir and heat to 300 °C under nitrogen atmosphere, and keep for 1 h to obtain solution e, cool to room temperature, add 41 mL of ethanol for flocculation, and magnetically separate 3 After three times, 10 mg of the precipitate was dispersed in 5 mL of dic...

Embodiment 1

[0078]Characterize the relaxation rate of the superparamagnetic iron oxide nanoparticle contrast agent prepared in Example 1 (Congo Red-MNPs) and Comparative Example 1 (MNPs), the method is as follows, the sample is prepared into 5 samples with different iron ion concentrations , take 200 μL each in the test tube, use the MQ60 magnetic resonance tester at room temperature, set the Carr-Purcell-Meiboom-Gill pulse sequence to measure the spin-spin relaxation time T of the sample 2 , with iron ion concentration and relaxation rate R 2 (1 / T 2 ) to make a linear regression curve, the calculated slope is the relaxation efficiency.

[0079] The result is as image 3 As shown, the Congo red modified superparamagnetic iron oxide nanoparticle contrast agent (Congo red-MNPs) prepared in Example 1 and the superparamagnetic iron oxide nanoparticle contrast agent (MNPs) not connected to the targeting molecule prepared in Comparative Example 1 ) relaxation rate shows a good linear relatio...

Embodiment 2

[0082] In this embodiment, the superparamagnetic iron oxide nanoparticle contrast agent is prepared by the following method, which specifically includes the following steps:

[0083] (1) Weigh 0.8g ferric oxalate and dissolve it in a mixed solvent of 20mL distilled water and 60mL ethanol to form solution a (concentration of solution a is 10mg / mL);

[0084] (2) Weigh 5.17g of sodium oleate and disperse it in 80mL of n-hexane to form solution b (that is, the mass fraction of oleate is 8.5wt%);

[0085] (3) After mixing solutions a and b, heat to 50°C, stir and reflux for 2h. After washing with water, dry in vacuum to obtain solid d;

[0086] (4) Dissolve 1 g of solid d in 10 mL of octadecene, add 1.0 mL of oleic acid, stir and heat to 280°C under nitrogen atmosphere, keep for 1 h to obtain solution e, cool to room temperature, add 44 mL of ethanol for flocculation, and magnetically separate 3 After three times, 10 mg of precipitate was dispersed in 10 mL of chloroform to obtai...

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Abstract

The invention provides superparamagnetism nanoparticles, a preparation method therefor and applications. Each superparamagnetism nanoparticle comprises an oleic-acid-coated ferroferric oxide nanoparticle located in the core, PEG-modified lipid molecules coating the surface of the oleic-acid-coated ferroferric oxide nanoparticle and target molecules connected with PEG. PEG-modified lipid molecules are employed as a modification agent, iron-containing compounds are subjected to thermal decomposition, then the target molecules are connected onto PEG, and a superparamagnetism nanoparticle is obtained. The superparamagnetism nanoparticles raise stability and biocompatibility of magnetic nanoparticles in vivo. Through connection of the target molecules, the nano particle diameter of the product is decreased further, the particle size distribution scope is narrowed, further the stability of the product is raised, the targeting of the product is raised, and imaging effects of a formed superparamagnetism contrast agent in lesion tissues of the Alzheimer disease are improved.

Description

technical field [0001] The invention belongs to the field of nanotechnology, and relates to a superparamagnetic nano particle, a preparation method and application thereof. Background technique [0002] MRI (Magnetic Resonance Imaging, MRI) is an imaging technique that uses the signal generated by the resonance of atomic nuclei in a magnetic field to reconstruct the image. Magnetic resonance imaging (MRI), as a new medical imaging diagnostic technology, has developed rapidly in recent years. The amount of information provided by MRI is not only more than that of many other imaging techniques, but also has great potential advantages in diagnosing diseases with the unique information it provides. It has outstanding advantages such as high resolution, many imaging parameters, and safe use. Better display of the structure of tissues and organs in the body and the nature and functional status of lesions can greatly improve the accuracy and early diagnosis. However, when the mag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/12A61K49/18
Inventor 张欣代凤英胡冰冰
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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