A kind of separation and extraction method of squalene in mixed tocopherols

Abstract

The invention provides a method for separating and extracting squalene in mixed tocopherols. The method comprises steps of: filling silochrom with the meshes of 100-200 into a chromatographic column, adopting an elution solvent to elute and compact the silochrom, then feeding the tocopherols into the chromatographic column according to a proper ratio, and separating the squalene from natural vitamin E through utilizing column chromatography. By adopting the separating and extracting method, the technological process is simple, the purity and recovery rate of the squalene can be improved to the maximum extent through utilizing a gradient elution method, silicagel columns can be well regenerated through utilizing strong polar solvents such as methyl alcohol and the like, and the method has the advantages of large solvent usage, easiness in recycling, small consumption and small environmental pollution.

Description

technical field [0001] The invention relates to a method for separating and extracting squalene, in particular to a method for separating and extracting squalene from natural vitamin E. Background technique [0002] Vitamin E (VitaminE), also known as tocopherol, refers to a class of substances with α-tocopherol biological activity. It is a fat-soluble vitamin that is easily soluble in organic solvents such as fat and ethanol, but insoluble in water. Stable to acid, unstable to alkali, sensitive to oxygen, insensitive to heat, it is one of the most important antioxidants. The most important physiological function of tocopherol to the human body is to promote reproduction. It can promote the secretion of sex hormones, protect T lymphocytes, protect red blood cells, resist free radical oxidation, inhibit platelet aggregation, and reduce the risk of myocardial infarction and cerebral infarction. It also has a good improvement effect on burns, frostbite, capillary bleeding, me...

AI Technical Summary

Problems solved by technology

[0005] The saponification separation method has an obvious effect of removing fatty acids and glycerides at one time, and significantly increases the content of squalene, but the operation process is complicated, and a large amount of lower alcohol is required in the reaction process, and it is carried out under alkaline conditions, which is very destructive to the target product;

[0006] Molecular distillation is especially suitable for laboratory separation and is also easy to industrialize, but the cost is high, and the content of the target product in the raw material is low, the extraction effect is not obvious, and pretreatment is required;

[0007] The supercritical extraction method is direct extraction, which is environmentally friendly and non-polluting, but the components in the raw materials have high solubility in supercritical carbon dioxide, so the separation efficiency is not high, and the investment and operating costs are relatively large;

[0008] The adsorption separation method has good separation effect and high product purity, which is suitable for product purification, but the separation scale is small, the separation cost is high, some eluents are easy to pollute the product, and the regeneration of the adsorbent is difficult;

[0009] The solvent extraction process and equipment are simple, and the operating cost is low, but the polarity of squalene in natural products is very small, the enrichment effect of the solvent extraction method is obvious, and the amount of solvent used is large (6-10 times the volume of the raw material), resulting in difficulty in recovery and environmental pollution