Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Florescent ion probe reagent for zinc ion detection and preparation method thereof

A technology of fluorescent ions and zinc ions, which is applied in the field of fluorescent ion probes for metal ion detection, can solve the problems of low water solubility, etc., and achieve the effects of easy monitoring, simple synthesis route, simple and practical identification operation

Inactive Publication Date: 2013-08-14
NANKAI UNIV
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many probes capable of detecting zinc ions have been reported, but few are water soluble and not interfered by cadmium ions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Florescent ion probe reagent for zinc ion detection and preparation method thereof
  • Florescent ion probe reagent for zinc ion detection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] Add 481mg (2mmol) of 2,9-dimethylol-1,10-phenanthroline to 20mL of anhydrous N,N'-dimethylformamide, cool to 0°C in an ice bath, add 100mg (4.2mmol) Sodium hydride, reacted under ice bath for 30min, added 500ul (4mmol) 3-bromopropyne and reacted under ice bath for 3 hours to obtain a reaction solution, then added 20mg (0.8mmol) sodium hydride to the reaction solution, at 18-25 React at ℃ for 12 hours, distill off the solvent at 0.1Pa to obtain solid a, dissolve the obtained solid a in 100ml of chloroform, extract three times with 50ml of water, dry the organic phase with anhydrous magnesium sulfate, Concentrate by distillation, and then use the mixed solution of dichloromethane and ethyl acetate with a volume ratio of 5:3 as the developing solvent, and separate it with a 200-300 mesh silica gel column to obtain 2,9-diargyl-1,10 - phenanthroline intermediate;

[0027] 2) Synthesis of bridged bis-β-cyclodextrin containing triazole phenanthroline

[0028] Under nitrogen ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a florescent ion probe reagent for zinc ion detection, which contains triazole phenanthroline bridge type bis(beta-cyclodextrin), adopts phenanthroline as a probe molecule and takes the nitrogen atom on the phenanthroline ring and the nitrogen atom on the triazole ring as bonding sites of bivalent zinc ions. A preparation method comprises the following steps of: firstly synthesizing a 2,9-dipropargyl-1,10-phenanthroline intermediate from 2,9-dihydroxymethyl-1,10-phenanthroline, 3-propargyl bromide and the like; and then synthesizing a target product from the intermediate, uni-6-deoxy-6-azido-beta-cyclodextrin, sodium ascorbate and the like. As a water-soluble zinc ion florescent probe, the synthesis path of the cyclodextrin derivative is simple, the production cost is low, and the florescent ion probe reagent is suitable for amplification synthesis and practical production application; the zinc ion identifying operation is simple and practical and has high sensitivity, and the zinc ion is displayed by a fluorescence spectrum; and as a zinc ion florescent probe, the florescent ion probe reagent disclosed by the invention can effectively detect zinc ions in cells and has broad application prospects.

Description

technical field [0001] The invention relates to the field of fluorescent ion probes for metal ion detection, in particular to a fluorescent ion probe reagent for zinc ion detection and a preparation method thereof. Background technique [0002] Zinc ion is an important metal ion in the human body, its content in the body is second only to iron ion, and the content of zinc in the adult body is about 2-3g. Zinc plays a very important role in organisms. Many biological processes such as brain function, pathology, gene transfection, metalloenzyme regulation, immune function, and central nervous system signal transmission are inseparable from the participation of zinc ions. Therefore, the detection of zinc ions, especially the determination of zinc in living cells and tissues, has been a field of great concern to biochemists in recent years. But since the zinc ion 3d 10 4s 0 Electronic layer structure, its own measurable spectrum and NMR signals, the commonly used ultraviolet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C09K11/06C08B37/16G01N21/64
Inventor 刘育张玉辉张瀛溟
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com