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Selenized microbial exopolysaccharide, and preparation method and uses thereof

An extracellular polysaccharide and microorganism technology, which is applied in the field of selenized small-molecule microbial exopolysaccharide and its preparation, can solve the problems of long reaction time, complicated reaction process, complicated preparation process, etc., and achieves no toxic by-product formation and tumor inhibition. The effect of high rate and simple reaction process

Inactive Publication Date: 2012-08-15
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN101024664A discloses a selenium galactose and its chemical synthesis. The method is to react galactose with an organic selenide reagent, the preparation process is complicated, and the monosaccharide does not have the pharmacological activity that natural polysaccharides have
[0005] The selenization technology mentioned in the above patents has the disadvantages of long reaction time, many reaction by-products, generally low selenium content, complex reaction process, and poor operability.

Method used

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  • Selenized microbial exopolysaccharide, and preparation method and uses thereof
  • Selenized microbial exopolysaccharide, and preparation method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Weigh 1.0 gram of small-molecule Caragana rhizobium exopolysaccharide (7.216kD) into the flask, then add 100 mL of nitric acid solution with a mass concentration of 1% to dissolve it, and stir at room temperature for 12 hours; then weigh 0.5 gram of Selenic acid and 0.6 g of barium chloride were added to the flask, stirred and heated at 60°C for 6 hours;

[0017] 2) Add 2.88 mL of 1 mol / L sulfuric acid dropwise to the above reaction solution, and centrifuge at 6000 rpm for 15 minutes to remove the precipitate;

[0018] 3) Use sodium hydroxide to adjust the pH value of the supernatant to 7, put it into a dialysis bag with a molecular weight cut-off of 3000Da, first dialyze with tap water for 60 hours, then dialyze with distilled water for 24 hours, until the dialysis is detected with 0.002 mg / mL ascorbic acid solution until the liquid outside the bag no longer turns red;

[0019] 4) Concentrate the dialysate under reduced pressure at 60°C and 0.08Mpa to 1 / 2 of the or...

Embodiment 2

[0021] 1) Weigh 1.0 gram of small-molecule Caragana rhizobium exopolysaccharide (10.352kD) into the flask, then add 100 mL of nitric acid solution with a mass concentration of 1% to dissolve it, and stir at room temperature for 8 hours; Selenic acid and 0.9 g of barium chloride were added into the flask, stirred and heated at 70°C for 7 hours;

[0022] 2) Add 4.32 mL of 1 mol / L sulfuric acid dropwise to the above reaction solution, and centrifuge at 6000 rpm for 20 minutes to remove the precipitate;

[0023] 3) Use sodium hydroxide to adjust the pH value of the supernatant to 7, put it into a dialysis bag with a molecular weight cut-off of 3000Da, first dialyze with tap water for 48 hours, then dialyze with distilled water for 24 hours, until the dialysis is detected with 0.002 mg / mL ascorbic acid solution until the liquid outside the bag no longer turns red;

[0024] 4) Concentrate the dialysate under reduced pressure at 60°C and 0.08Mpa to 1 / 2 of the original volume, add 20...

Embodiment 3

[0026] 1) Weigh 1.0 gram of small molecule Caragana rhizobium exopolysaccharide (30.641kD) and add it to the flask, then add 100 mL of nitric acid solution with a mass concentration of 2% to dissolve it, and stir at room temperature for 10 hours; Selenic acid and 1.2 grams of barium chloride were added to the flask, stirred and heated at 80°C for 6 hours;

[0027] 2) Add 5.76 mL of 1 mol / L sulfuric acid dropwise to the above reaction solution, and centrifuge at 5000 rpm for 15 minutes to remove the precipitate;

[0028] 3) Use sodium hydroxide to adjust the pH value of the supernatant to 7, put it into a dialysis bag with a molecular weight cut-off of 5000Da, first dialyze with tap water for 72 hours, then dialyze with distilled water for 36 hours, until the dialysis is detected with 0.001 mg / mL ascorbic acid solution until the liquid outside the bag no longer turns red;

[0029] 4) Concentrate the dialysate under reduced pressure at 60°C and 0.08Mpa to 1 / 2 of the original vo...

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Abstract

The invention provides a selenized microbial exopolysaccharide and a preparation method thereof. The preparation method comprises the steps of: adding micromolecular Rhizobium sp. exopolysaccharide to nitric acid solution, stirring, adding selenous acid and barium chloride, adding sulfuric acid dropwise, removing precipitates by centrifugation, regulating the pH of supernatant to 7 by sodium hydroxide, putting the supernatant in a dialysis bag for dialysis, concentrating the dialysate under reduced pressure and then adding edible alcohol, separating out selenized microbial exopolysaccharide, and collecting and freeze-drying the selenized microbial exopolysaccharide to obtain selenized micromolecular microbial exopolysaccharide. The content of selenium in the exopolysaccharide can reach 586 to 790 mu g / g according to measurement; mice tests show that the exopolysaccharide has excellent antitumor activity; and the exopolysaccharide can be used in preparing antitumor medicines and healthcare products.

Description

technical field [0001] The invention relates to the technical field of selenized polysaccharides, in particular to a selenized small molecular microbial extracellular polysaccharide and its preparation method and application. Background technique [0002] Biological polysaccharides widely exist in nature and participate in life activities such as energy transfer, development and differentiation, and immune regulation. There are many types of polysaccharides, which have physical and chemical properties such as flocculation, film-forming, gelling, biosafety, biodegradability, and biocompatibility; , lowering blood pressure and many other aspects have good pharmacological activity. Structural factors that affect the biological activity of polysaccharides include the nature of the main chain of polysaccharides, the nature of branched chains, and the high-level structure of polysaccharide molecules; the composition of sugar units in the main chain of polysaccharides directly det...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A23L1/29A23L33/00
Inventor 赵良启聂瑞红吕利华刘坚
Owner SHANXI UNIV
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