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Extraction method of cynanchum otophyllum saponin

A technology of Qingyang ginseng saponin and extraction method, which is applied in the field of natural product separation, can solve the problems of difficult dispersion of concentrated liquid, blockage of macroporous resin, poor water solubility, etc., and achieve the effects of small product loss, easy amplification, and short cycle

Inactive Publication Date: 2012-05-23
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the total glycosides of Qingyang ginseng are mostly steroidal saponins with poor water solubility, the concentrated solution is difficult to disperse in water, easy to precipitate, block the macroporous resin, difficult to put on the column, and cause serious pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 1 kg of Qingyang ginseng, grind it into 40 meshes, dissolve 2 g of cellulase and 2 g of amylase in 100 ml of aqueous hydrochloric acid at pH 4, add it to the raw material, mix well, and perform enzymatic hydrolysis at room temperature for 36 hours to obtain the raw material for enzymatic hydrolysis. Put the enzymolysis raw material into a microwave extraction tank, add 10L90% methanol solution to extract for 30 minutes, filter the extract, add 10L90% methanol solution to extract for 30 minutes, combine the two filtrates, filter through a 400-mesh filter, and add the filtrate with a molecular weight cut-off of 6000 Tubular composite membrane ultrafiltration, the pressure is controlled at 5kpa, the permeate is collected, the filtrate is concentrated under reduced pressure, and an appropriate amount of water is added to obtain a concentrated solution of 150ml. Add 300ml of water-saturated n-butanol to the concentrated solution and stir evenly, pour it into a separatory...

Embodiment 2

[0028] Take 1 kg of Qingyang ginseng, crush it into 60 meshes, dissolve 3 g of cellulase and 1 g of amylase in 100 ml of sulfuric acid aqueous solution at pH 5, add it to the raw material, mix well, and perform enzymatic hydrolysis at room temperature for 54 hours to obtain the raw material for enzymatic hydrolysis. Put the enzymolysis raw material into a microwave extraction tank, add 10L90% ethanol solution to extract for 30 minutes, filter the extract, add 10L90% ethanol solution to extract for 30 minutes, combine the two filtrates, filter through a 500-mesh filter, and add 10000 molecular weight cut-off to the filtrate Tubular composite membrane ultrafiltration, the pressure is controlled at 6kpa, the permeate is collected, the filtrate is concentrated under reduced pressure, and an appropriate amount of water is added to obtain a concentrated solution of 200ml. Add 400ml of water-saturated n-butanol to the concentrated solution and stir evenly, pour it into a separatory fu...

Embodiment 3

[0030] Take 1 kg of Qingyang ginseng, crush it into 80 meshes, dissolve 1 g of amylase and 2 g of pectinase in 100 ml of aqueous hydrochloric acid at pH 3, add it to the raw materials, mix well, and perform enzymatic hydrolysis at room temperature for 36 hours to obtain the raw materials for enzymatic hydrolysis. Put the enzymatic hydrolysis raw material into a microwave extraction tank, add 10L95% ethanol solution for extraction for 30 minutes, filter the extract, add 10L95% ethanol solution for extraction for 30 minutes, combine the two filtrates, filter through a 500-mesh filter, and add the filtrate with a molecular weight cut-off of 6000 Tubular composite membrane ultrafiltration, the pressure is controlled at 5kpa, the permeate is collected, the filtrate is concentrated under reduced pressure, and an appropriate amount of water is added to dissolve to obtain a concentrated solution of 120ml. Add 300ml of water-saturated n-butanol to the concentrated solution, heat and sti...

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PUM

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Abstract

The invention relates to an extraction method of cynanchum otophyllum saponin. In the extraction method, biological enzymolysis, membrane separation and an aluminum oxide short column are adopted to purify cynanchum otophyllum saponin, thus the product has high yield and high content. The extraction method comprises the following specific steps of: crushing cynanchum otophyllum into 40-80 meshes, adding 0.2-0.5% a bioenzyme for natural enzymolysis to obtain an enzymolysis raw material, adding low-carbon alcohol solution, the amount of which is 5-10 times that of the enzymolysis raw material, to the enzymolysis raw material, carrying out microwave extraction 2-3 times, adding the extract to an ultrafiltration membrane for filtering, concentrating the filtrate under reduced pressure, adding water-saturated n-butyl alcohol to the concentrated solution and extracting for 2-3 times, collecting n-butyl alcohol layer, concentrating and recovering a reagent, adding a little ethyl ether to the concentrated solution for precipitating, filtering out the precipitate, adding 95% ethanol for refluxing and dissolving, passing the dissolved liquid through an aluminum oxide-activated carbon mixed short column, recovering ethanol from effluent, and drying under reduced pressure to obtain the cynanchum otophyllum saponin. The extraction method provided by the invention is simple in technological operation, low in energy consumption and low in pollution, and is suitable for industrial production.

Description

Technical field: [0001] The invention relates to the field of separation of natural products, in particular to a method for extracting ginseng saponins by enzymatic hydrolysis and membrane separation. Background technique: [0002] Qingyang ginseng is a plant of the genus Geshanxiao in the family Romoaceae. The Yi medicine is called Roujiboqi, and the Chinese medicine is called Qingyang ginseng, Xihe ginseng, Xiaobai ginseng, and bone ginseng. It is also called Naogou medicine in Yunnan. Distributed in Yunnan, Guizhou, Hunan, Guangxi and other places, the roots are used as medicine in autumn. "Yi Yao Zhi" records: nourishing the kidney and strengthening tendons, invigorating the spleen and harmonizing the stomach, expelling wind and dampness, detoxification, sedation, and deworming. "Southern Yunnan Materia Medica" records: folks use it to strengthen the waist, strengthen the kidneys, and strengthen the muscles and bones. [0003] Modern medicine shows that the main medici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/27A61P1/14A61P25/04A61P25/08
Inventor 李法庆杨成东刘东锋
Owner 苏州宝泽堂医药科技有限公司
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