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Gradient active scaffold material for osteochondral repair and its preparation method and application

A scaffold material, osteochondral technology, applied in medical science, prosthesis and other directions, can solve the problems of poor repair ability, low metabolic rate physiological characteristics, disease transmission and other problems, and achieve the effect of simple preparation method, firm adhesion and adhesion promotion

Inactive Publication Date: 2011-12-14
SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, adult articular cartilage lacks direct blood supply and innervation, and has the physiological characteristics of low metabolic rate, and its self-repair ability is extremely poor. Cartilage defects with a diameter greater than 2 mm can hardly be completely repaired. If combined with subchondral bone defects, it is more difficult to treat
There are obvious disadvantages in the methods for the treatment of osteochondral injuries in clinical application: (1) Oral medicine, intra-articular injection of hyaluronic acid, joint lavage and debridement, etc., can only relieve symptoms and delay the progress of the disease , unable to repair damaged cartilage and subchondral bone
(2) Drilling, microfracture and other operations can use a small amount of MSCs that naturally infiltrate into the drilling area through the medullary cavity without special treatment to repair the defect, but the new stimulation is scar tissue and type I collagen. The main fibrocartilage does not have the biomechanical properties of normal articular cartilage, and is far from meeting the needs of articular cartilage mechanics
(3) Autologous chondrocyte transplantation or autologous osteochondral block chimeric transplantation has achieved certain therapeutic effects, but the source of materials is limited, the donor is insufficient, the expansion ability of chondrocytes is not good, and it causes damage to the donor site, leaving scars, etc. The reason greatly limits its application
(4) Allogeneic osteochondral transplantation, although it can provide the donor material at the corresponding position, but there are disadvantages such as immune rejection, incomplete donor-recipient fusion, cartilage separation, and the risk of disease transmission
It is generally believed that the pore size must be greater than 100 μm, otherwise the tissue can only invade the surface of the implant, and it is difficult to obtain sufficient nutrients in the tissue

Method used

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  • Gradient active scaffold material for osteochondral repair and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0052] (1) Type I collagen, calcium nitrate tetrahydrate, and diammonium hydrogen phosphate are used as raw materials, and ammonia water is used to adjust PH=8.0, and HA / Col powder is prepared by coprecipitation method. In the prepared HA / Col-I composite powder, Type I collagen content is 35% and hydroxyapatite content is 65%;

[0053] (2) Use the EDC / NHS-Heparin method to construct a controlled release system for biologically active substances, and soak the HA / Col-I composite powder and the previously prepared type II collagen powder in 0.05mol / LMES buffer (pH 5.6) 30min for standby, dissolve each 2.5g of heparin in 300ml of 0.05mol / L MES buffer containing 2gEDC and 1.5gNHS for activation for 10min, put the spare collagen into the MES containing heparin and EDC / NHS according to the weight ratio of collagen to heparin 1:2 In the buffer solution, gently shake the reaction at a constant temperature of 37°C for 4h; then place the modified HA / Col-I composite powder and type II col...

Embodiment 2

[0057] (1) With type I collagen, calcium nitrate tetrahydrate, and diammonium hydrogen phosphate as raw materials, ammonia water is used to adjust the pH value to 8.0, and HA / Col powder is prepared by co-precipitation method. In the prepared HA / Col-I composite powder , the content of type I collagen is 20%, and the content of hydroxyapatite is 80%;

[0058] (2) Use the EDC / NHS-Heparin method to construct a controlled release system for biologically active substances, and soak the HA / Col-I composite powder and the previously prepared type II collagen powder in 0.05mol / LMES buffer (pH 5.6) 30min for standby, dissolve every 3g of heparin in 200ml of 0.05mol / L MES buffer containing 1gEDC and 2gNHS for activation for 10min, put the spare collagen into the MES buffer containing heparin and EDC / NHS according to the weight ratio of collagen to heparin 1:3 Gently shake and react for 4 hours at a constant temperature of 37°C; then place the modified HA / Col-I composite powder and type II...

Embodiment 3

[0062] (1) With type I collagen, calcium nitrate tetrahydrate, and diammonium hydrogen phosphate as raw materials, ammonia water is used to adjust the pH value to 8.0, and HA / Col powder is prepared by co-precipitation method. In the prepared HA / Col-I composite powder , the content of type I collagen is 50%, and the content of hydroxyapatite is 50%;

[0063] (2) Use the EDC / NHS-Heparin method to construct a controlled release system for biologically active substances, and soak the HA / Col-I composite powder and the previously prepared type II collagen powder in 0.05mol / LMES buffer (pH 5.6) 30min for standby, dissolve each 1g of heparin in 500ml of 0.05mol / L MES buffer containing 3gEDC and 0.5gNHS for activation for 10min, put the spare collagen into the MES buffer containing heparin and EDC / NHS according to the weight ratio of collagen to heparin 1:1 solution, and gently shaken at a constant temperature of 37°C for 4 hours; then the modified HA / Col-I composite powder and type II...

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Abstract

The invention relates to a gradient active scaffold material for osteochondral repair and its preparation method and application. The active scaffold material is mainly composed of a cartilage layer, a calcified layer and a subchondral bone layer respectively loaded with biologically active substances, wherein the calcified layer is placed Between the cartilage layer and the subchondral bone layer, between the cartilage layer and the calcified layer, and between the calcified layer and the subchondral bone layer are organically connected by a biomedical degradable polymer; the cartilage layer, the calcified layer and the subchondral bone layer It has interpenetrating pores; its preparation method is: (1) preparation of HA / Col-I composite powder; (2) construction of bioactive substance controlled release system; (3) molding preparation of gradient active scaffold material for osteochondral repair The active scaffold material is used to prepare osteochondral complex; the active scaffold material can effectively promote the adhesion, proliferation and differentiation of cells, and is suitable for the repair and reconstruction of bone and cartilage and the delivery of nutrients.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a gradient active scaffold material for osteochondral repair and its preparation method and application. Background technique [0002] Osteochondral defects in joints are very common in clinical practice, often leading to joint pain, limited mobility, osteoarthritis, and even limb dysfunction and disability, which seriously affect the quality of life of patients and have become one of the main causes of physical disabilities. one. However, adult articular cartilage lacks direct blood supply and innervation, has the physiological characteristics of low metabolic rate, and has extremely poor self-repair ability. Cartilage defects with a diameter > 2 mm can hardly be completely repaired. If combined with subchondral bone defects, it is more difficult to treat. There are obvious disadvantages in the methods for the treatment of osteochondral injuries in clinical application:...

Claims

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Application Information

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IPC IPC(8): A61L27/40C08J9/26
Inventor 李志宏武继民郭涛张西正关静李瑞欣郭勇黄姝杰侍才洪马军
Owner SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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