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Method for preparing mesh-shaped bionic bone porous stent material

A technology of porous scaffold and bionic bone, which is applied in the field of biomedical materials and tissue engineering regenerative medicine, can solve problems such as unsatisfactory practical application effects, excessively large pore structure, and application research limitations, and achieve significant social and economic benefits. Preparation Simple process and broad application prospects

Inactive Publication Date: 2011-05-18
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the preparation method of the bionic bone scaffold that is concentrated in research and patent reports is deorganic matter / cell animal bone [Yu Fangyuan, Huang Lihong, Ma Yuanzheng, etc. A tissue engineered osteochondral scaffold and its preparation method. Patent application number: CN101822851A and Bi L, Li D, Liu M, et al.The influence of approaches for the purification of natural cancellous bone grafts: Morphology, microstructure, composition, strength and biocompatibility study. Mater Lett, 2010, 64: 2056-2059], but there is an immune Risk of pathogenicity and infectious diseases; the pore size of bionic scaffolds prepared from bones such as starfish and sea urchins is usually only tens of microns, which is quite different from the ideal pore size of 100-400 microns required for bone tissue engineering scaffolds, and the actual application effect is not ideal; coral The porous structure (including morphology and pore size distribution) of bone is similar to the structure of natural bone pores, but its chemical composition is mainly calcium carbonate [Wu YCh, Lee TM, Chiu KH, et al. A comparative study of the physical and mechanical properties of three natural corals based on the criteria for bone-tissue engineering scaffolds. J Mater Sci: Mater Med, 2009, 20: 1273-1280], so that its application in bone tissue engineering is greatly limited; and plant tissues such as loofah and wood directly For bone tissue engineering, there are disadvantages such as excessively large pore structure (millimeter and centimeter) and lack of biological activity.

Method used

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  • Method for preparing mesh-shaped bionic bone porous stent material
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  • Method for preparing mesh-shaped bionic bone porous stent material

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1: Preparation of Biomimetic 45S5 Bioactive Glass Scaffold

[0029] 1) Template treatment: first soak the natural sponge template in 0.5mol / L acetic acid aqueous solution for 24 hours, and stir slightly during the period. After soaking, take out the natural sponge template and rinse it with distilled water for 3 times; then, rinse the natural sponge template with distilled water Immerse the template in 0.5mol / L sodium hydroxide aqueous solution, keep it under stirring for 24 hours, wash the lye with distilled water after taking it out; finally, dry it under vacuum at 45°C; see the treated natural sponge template figure 1 ,Depend on figure 1 It can be seen that the natural sponge has a typical mesh-like pore structure, the pore size is mainly concentrated in the range of 100-300 μm, the average diameter of the porous skeleton is 20 μm, the skeleton is connected in a trident form, and the porosity is high.

[0030] 2) Preparation of 45S5 sol: firstly mix absolute...

Embodiment 2

[0034] Example 2: Preparation of Biomimetic Hydroxyapatite Scaffold

[0035] 1) Template treatment: first soak the natural sponge template in 2mol / L acetic acid aqueous solution for 12 hours, stir slightly during the period, take out the natural sponge template and rinse it with distilled water for 5 times after soaking; then, rinse the natural sponge template with distilled water Immerse in 2mol / L sodium hydroxide aqueous solution, keep it under stirring for 36 hours, take it out and wash the lye with distilled water; finally, dry it under vacuum at 60°C;

[0036] 2) Preparation of hydroxyapatite water-based slurry: adding nano-hydroxyapatite powder in batches to distilled water containing 5% by mass of binder polyvinyl alcohol and 1% of dispersant ammonium polyacrylate, and dissolving Adjust the pH value of the water-based slurry to 10, and then undergo ball milling at 400 rpm for 8 hours to make a hydroxyapatite water-based slurry with a solid content of 55 wt%.

[0037] 3...

Embodiment 3

[0040] Example 3: Preparation of biomimetic β-tricalcium phosphate scaffold

[0041] 1) Template treatment: first soak the natural sponge template in 1mol / L acetic acid aqueous solution for 36 hours, stir slightly during the period, take out the natural sponge template and rinse it with distilled water for 3 times after soaking; then, rinse the natural sponge template with distilled water Immerse in 1.5mol / L sodium hydroxide aqueous solution, keep it under stirring for 24 hours, take it out and wash the lye with distilled water; finally, vacuum dry at 80°C;

[0042] 2) Preparation of tricalcium phosphate sol: firstly mix absolute ethanol and water at a volume ratio of 3:7 and adjust the pH value of the mixed solution to 1 with nitric acid to obtain ethanol-water solution, then use triethyl phosphate and calcium nitrate as raw materials , measure the raw materials according to the chemical composition ratio of tricalcium phosphate, add the measured raw materials into the ethano...

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Abstract

The invention relates to a method for preparing mesh-shaped bionic bone porous stent material, comprising the following steps: taking a multi-cellular marine animal natural sponge processed by organic acid and inorganic alkali solution as a template, impregnating with sol or water based slurry of hydroxylapatite, 45S5 bioactive glass, beta-tricalcium phosphate or calcium silicate, drying, oxidizing, demoulding and sintering at high temperature, thereby preparing the bone tissue engineering stent material in a mesh-shaped pore structure. The pore structural parameters and degradation performance of a porous stent are controlled by adjusting the solid content in the sol or the slurry, impregnation frequency and parameters in the process of oxidizing and demoulding as well as sintering at high temperature. Pore size of the mesh-shaped porous stent produced by adopting the invention is 100-300Mum, porosity is 68-94%, and the porous stent is highly similar to a natural cancellous bone in the shape and pore size. The product prepared by adopting the invention has the characteristics that bionic degree of the porous structure is high, adaptation to materials is high, process is simple and cost is low and the product has a great application potential in the regeneration repairing technology of hard tissue engineering, such as bone, basibranchial bone and teeth.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and tissue engineering regenerative medicine, and relates to a preparation method of a bionic porous support material for bone tissue engineering, in particular to a preparation method of a mesh-shaped bionic bone porous support material. Background technique [0002] Tissue engineering is a new type of regenerative medical technology developed in recent years for the purpose of repairing, maintaining and promoting the shape, structure and function of defective tissues or organs. This technology achieves tissue or organ regeneration and repair by planting seed cells in a three-dimensional porous scaffold material, culturing, proliferating and differentiating in vitro or in vivo, and is expected to replace autologous tissue transplantation, allogeneic / xenogeneic tissue transplantation and Restorative methods such as implants. In tissue engineering technology, the three-dimensional por...

Claims

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Application Information

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IPC IPC(8): A61L27/12A61L27/10A61L27/02A61L27/56A61K6/033A61K6/027A61K6/838
Inventor 钱军民徐明辉乔冠军
Owner XI AN JIAOTONG UNIV
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