Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Exogenous cornea substrate without cells and its preparation method and use

A corneal stroma, cell-free technology, used in medical science, eye implants, prostheses, etc., can solve the problem of infectious pathogens infecting the human body

Inactive Publication Date: 2007-08-29
THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the strong rejection after xenotransplantation and the possibility of infectious pathogens infecting the human body after xenotransplantation have prevented xenotransplantation from being clinically applied so far. 8

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Exogenous cornea substrate without cells and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of decellularized porcine corneal stroma

[0024] Choose healthy donor pigs from qualified breeding farms, use thiopental sodium needles for intramuscular injection anesthetize the pig’s eyeballs, trim the eyeball surface appendages, soak the eyeballs in 5% povidone-iodine for 3 minutes, and then use Rinse with 500 ml of normal saline and store it in -70°C frozen until decellularization.

[0025] After the eyeball is thawed at room temperature, cut the whole cornea along the limbus under an operating microscope, scrape off the corneal epithelial layer and tear off the elastic membrane, see Figure 1a, Figure 2a, Figure 3a, and then place the corneal stroma in 30ml ionotype In the scale agent, place it on a constant temperature shaker at 300 rpm for 24 hours without interruption, and change the solution every 6 hours. The ionic detergent is dissolved in 0.05% SDS (SIGMA chemical company, St. Louis, MO). Ionized water with 1mmol / L EDTA added, the treatmen...

Embodiment 2

[0028] Example 2 Preparation of acellular bovine corneal stroma

[0029] Select healthy donor cows in qualified breeding farms, anesthetize with thiopental sodium needle intramuscular injection, remove the eyeballs, trim off the surface of the eyeballs and soak the eyeballs in 5% povidone-iodine for 3 minutes, and then use Rinse with 500 ml of normal saline and transport to the laboratory at 4 degrees Celsius.

[0030]Cut the whole cornea along the limbus under an operating microscope, scrape off the epithelial layer, tear off the posterior elastic membrane of the cornea, and then put the above-mentioned heterogeneous corneal stroma in 30ml non-ionic detergent and shake it continuously at 300 rpm for 72 hours. The solution is changed every 12 hours. The non-ionic detergent is 0.5% Triton X-100 (SIGMAchemical company, St. Louis, MO) dissolved in deionized water, and 25mmol / L EDTA (SIGMA chemical company, St. Louis) is added. , MO), the treatment temperature is 10°C, and the solutio...

Embodiment 3

[0033] Example 3 Therapeutic corneal transplantation experiment in New Zealand rabbits

[0034] The acellular bovine corneal stroma prepared by the method in Example 2 was used as the donor material (hereinafter referred to as the donor), and healthy New Zealand rabbits were selected as the recipients for therapeutic penetrating keratoplasty. The rabbits were successfully injected with thiopental sodium intramuscularly After general anesthesia, the surgical eye is routinely disinfected with PVP iodine, drape, eyelid opener to open the eyelid, upper and lower rectus fixation sutures are made, the donor cornea is flushed with saline, and the inner matrix is ​​placed on the corneal pad with a 7.5 mm ring. Drill in the center of the recipient’s cornea to the anterior chamber, and inject viscoelastic agent into the front. Use corneal scissors to cut the recipient’s cornea along the drill edge to prepare an implant bed. Place the implant on the implant bed and use 10-0 nylon thread (Alc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a cell-less heterogenetic cornea material and manufacturing method and use, the manufacturing method for the product is: acquires the fresh animals' cornea at first; eliminates the cell membrane of the cornea material and nucleic acid with chemical method and enzyme method; carries on dehydration process to the cell-less heterogenetic cornea material; stores for backup. The method eliminates the antigenicity of the heterogenetic cornea material through eliminating the cell elements in the cornea material. At the same time, it reserves the integrity of glue fiber and transparency of the cornea. The material can be applied to construction of manmade cornea or used as the medical material for cornea transplantation, and cornea refraction operation directly.

Description

Technical field [0001] The invention belongs to a medical material, and relates to a corneal matrix, in particular to an acellular heterogeneous corneal matrix and a preparation method. This acellular heterogeneous corneal matrix can be used for tissue engineering artificial cornea construction and as a medical material Corneal transplantation, refractive surgery. Background technique [0002] Corneal disease is the second leading cause of blindness worldwide. 1 For patients who are blinded by corneal diseases, only through corneal transplantation can the patients restore their vision. In my country, there are approximately 250,000 cases of blindness due to corneal diseases each year, while only a few thousand cases of corneal transplantation are received each year. The fundamental reason restricting the development of corneal transplantation is the extreme lack of donor cornea sources. Therefore, research and development of corneal substitutes that can replace human cornea is th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/14A61L27/36A61L27/54A61L27/00
Inventor 姚玉峰朱也飞裘文亚
Owner THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com