The invention discloses a connection structure of a cable and a printed circuit board (PCB). The connection structure comprises the PCB and more than one cable, wherein each cable is provided with a differential signal line pair, an insulation layer and a metal shielding jacket, the insulation layer wraps the differential signal line pair, the metal shielding jacket wraps the insulation layer, a substrate is arranged on the PCB, a GND layer is arranged in the substrate, a signal end and a GND BAR are arranged on a surface of the substrate, the GND BAR is connected to the GND layer in a conduction layer, and the metal shielding jacket of the cable is pasted onto the GND BAR by conductive glue so as to achieve electrical conduction of the metal shielding jacket and the GND BAR; or a grounding metal wire is arranged in the cable, the grounding metal wire extends out of an end part of the metal shielding jacket and is returned back, and a return-back part of the grounding metal wire is electrically connected with the GND BAR for conduction. Therefore, the GND BAR is arranged on the PCB, the grounding metal wire is connected with the GND BAR or the metal shielding jacket is circuit-shorted with the GND BAR to achieve characteristic impedance matching, and an unstable variable during manual operation is effectively eliminated.