The invention discloses a sampling room for COVID-19 nucleic acid detection, and belongs to the technical field of medical facilities. The sampling room comprises a base, a front wall, a back wall, two side walls and a top plate, wherein the base, the front wall, the back wall, the two side walls and the top plate are enclosed into a cuboid structure; a transparent window part is arranged in the middle of the front wall, the transparent window part is provided with a sampling hole group and antiviral gloves, the sampling hole group comprises a pair of sampling holes, the finger sides of the antiviral gloves are arranged outside the transparent window part, the cuff of the left glove of the antiviral gloves is hermetically installed in the sampling hole in the left side, and the cuff of theright glove of the antiviral gloves is hermetically installed in the sampling hole in the right side; the back wall is provided with an inlet and a door body, and one side edge of the door body is rotatably installed on one side of the inlet; and when medical staff are located in a closed containing cavity and a to-be-sampled person is located on the outer sides of the sampling holes, the medicalstaff can conduct sampling operation on the to-be-sampled person through the antiviral gloves. The sampling room for the COVID-19 nucleic acid detection has the advantages of convenience and safety in use, and the manufacturing cost and the protection level of the medical staff can be reduced.