46 results about "Withanolide" patented technology

Cyclooxygenase-2 enzyme inhibiting withanolides are described. In particular, compounds from Withania somnifera are the preferred source of the withanolides, although they can be from other plant sources. The COX-2 inhibition is selective over COX-1.

Novel isolated and purified withanamides and withanolides are described. In particular, compounds from Withania somnifera fruit are the preferred source of the withanamides and withanolides, although they can be from other plant sources. In addition to their use as powerful antioxidants, the withanamides and withanolides can be useful for the treatment of depression, Alzheimer's Disease, obesity and migraine headaches.

The present invention provides a Withania somnifera fraction rich in withanolides and a vaccine comprising a “Withania somnifera fraction” as an adjuvant.

The invention discloses a method for extracting withanolide. The method has the characteristics of high efficiency, little pollution and simple operation, and the steps are as follows: a. Take the raw material of Indian ginseng root and grind it into 40-80 mesh, put it in the extraction kettle, at the extraction pressure of 22-27MPa, and the temperature of 40-50°C, put in liquid CO2 and entrainer, the flow rate is 20-40L / h, and extract 2-3 After 1 hour, analyze the extract; b. Add water to disperse the recovery reagent of the above extract, add it to the macroporous resin for separation, ethanol solution gradient elution, the eluate is concentrated to a small volume of crystals, recrystallized with ethanol for 2-3 times, and dried Get withanide.

Disclosed is a method of enhancing the response of cancer cells in a mammal to treatment with a death receptor ligand, which method comprises contacting the cancer cells with a death receptor ligand in conjunction with an effective amount of a compound described herein, for example, a cucurbitacin (I) or a withanolide (II). Also disclosed is a method of inducing apoptosis in cancer cells in a mammal, comprising contacting the cancer cells with a compound described herein, for example, a cucurbitacin (I) or a withanolide (II) and also contacting the cancer cells with a death receptor ligand, whereby apoptosis is induced in the cancer cells.

Provided are methods for screening for drugs effective for treating fibrotic conditions. One screening method comprises exposing a cell to a test compound, monitoring the effect of the test compound on the amount or form of a cell molecule, comparing the amount or form of the cell molecule with the result obtained by treatment of the cell with an anti-fibrotic -effective amount of a withanolide compound, and selecting a drug effective for treating a fibrotic disease based on the ability of the test compound to provide the effect obtained by the withanolide compound on the cell molecule. Also provided is a method of treating a fibrotic disease comprising administering an anti-fibrotic effective amount of a withanolide compound to a mammal in need of treatment for fibrosis.

A method of treating tinnitus includes the step administering a composition to the mammal, wherein the composition consists essentially of a biologically effective amount of vitamin A, vitamin E, vitamin C, a vasodilator comprising magnesium, and, optionally, a withanolide, and / or resveratrol.

The invention discloses a method for extraction separation of a withanolides compound from waste Chinese wolfberry branches. The method comprises the steps of: (1) extracting waste Chinese wolfberry branches with ethanol, combining the extracted liquid, and recovering ethanol to obtain a concentrated solution; (2) adding ethyl acetate into the concentrated solution to perform extraction, recovering the organic phase and then performing concentration to obtain an extract; (3) diluting the extract, loading the diluted extract to a selective macroporous adsorption resin column, conducting elution with ethanol, recovering ethanol from the obtained eluent, and conducting crystallization with ethanol so as to obtain the withanolides compound; (4) loading the withanolides compound crystal to a silica gel column, performing elution with a mixed solution of n-hexane and ethyl acetate, carrying out segmented collection of the eluent, thus obtaining withanolides A and withanolides B respectively in order; and (5) recovering the solvent from the segmented eluent in step (4), and then performing crystallization with ethanol so as to obtain a withanolides A monomer and a withanolides B monomer respectively. The method provided by the invention has high extraction yield, and the obtained monomers have high purity.

A composition to be administered to a mammal for treating hearing loss is disclosed herein. The composition consists essentially of a biologically effective amount of vitamin A, vitamin C, vitamin E, and a vasodilator comprising magnesium. The composition optionally includes a withanolide and / or resveratrol. The vitamin A, vitamin C, vitamin E, and the vasodilator comprising magnesium provides an additive effect that is equal to or greater than a sum of the effects of the individual components.

Provided herein are synthetic analogs of withanolide natural products of formula (I), wherein R1-R4 are as defined herein, and their pharmaceutical uses in treating neurodegenerative diseases.

Cyclooxygenase-2 enzyme inhibiting withanolides are described. In particular, compounds from Withania somnifera are the preferred source of the withanolides, although they can be from other plant sources. The COX-2 inhibition is selective over COX-1.