A kind of extraction method of withanolide

A technology of withanolide and extraction method, which is applied in the field of natural medicinal chemistry, can solve the problems of low extraction efficiency, serious pollution, and low safety, and achieve the effects of shortening the extraction cycle, high product purity, and less dissolution of impurities

Inactive Publication Date: 2011-11-30
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in these existing methods, the reagents used have high toxicity, low safety, serious pollution, low extraction efficiency and long cycle time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The raw material of Indian ginseng root is crushed into 80 meshes, 1 kg is taken and placed in the extraction kettle, and liquid CO 2 and 95% ethanol (ratio 1:50), flow rate 40L / h, extract at extraction pressure 22MPa, temperature 40°C for 2 hours, adjust analysis temperature 50°C, pressure 10MPa, and collect the extract. After recovering ethanol from the extract, add water to disperse, use AB-8 macroporous resin column (resin volume: 200ml) for adsorption, first use 600ml of 50% ethanol solution for impurities, then take 600ml of 90% ethanol solution for elution, collect the eluent and concentrate it to 100ml and place it for crystallization , filtered out the crystals, crystallized twice with 90% ethanol, and dried to obtain 10 g of off-white withanide, with a content of 89%.

Embodiment 2

[0019] The raw material of Indian ginseng root is crushed into 60 meshes, 1 kg is taken and placed in the extraction kettle, and liquid CO 2 and 99% ethanol (ratio 2:50), flow rate 20L / h, extract at extraction pressure 27MPa, temperature 50°C for 3 hours, adjust analysis temperature 50°C, pressure 10MPa, and collect the extract. After recovering ethanol from the extract, add water to disperse it, then use HZ818 macroporous resin column (resin volume: 200ml) to absorb, first use 1000ml of 40% ethanol solution to remove impurities, then take 800ml of 80% ethanol solution to elute, collect the eluate and concentrate it to 150ml, place it for crystallization, filter After crystallization, crystallize with 99% ethanol for 3 times, and dry to obtain 7.5 g of off-white withanide, with a content of 92%.

Embodiment 3

[0021] The raw material of Indian ginseng root is crushed into 40 meshes, 1 kg is taken and placed in the extraction kettle, and liquid CO 2 and 99% ethanol (ratio 1:50), flow rate 25L / h, extract at extraction pressure 25MPa, temperature 45°C for 3 hours, adjust analysis temperature 40°C, pressure 5MPa, and collect the extract. After recovering ethanol from the extract, add water to disperse, then use HPD-100 macroporous resin column (resin volume: 200ml) to absorb, first use 800ml of 50% ethanol solution to elute the impurities, then take 900ml of 70% ethanol solution to elute, collect the eluent and concentrate it to 200ml and place it for crystallization , filtered out the crystals, crystallized twice with 90% ethanol, and dried to obtain 9 g of off-white withanide, with a content of 87%.

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Abstract

The invention discloses a method for extracting withanolide. The method has the characteristics of high efficiency, little pollution and simple operation, and the steps are as follows: a. Take the raw material of Indian ginseng root and grind it into 40-80 mesh, put it in the extraction kettle, at the extraction pressure of 22-27MPa, and the temperature of 40-50°C, put in liquid CO2 and entrainer, the flow rate is 20-40L / h, and extract 2-3 After 1 hour, analyze the extract; b. Add water to disperse the recovery reagent of the above extract, add it to the macroporous resin for separation, ethanol solution gradient elution, the eluate is concentrated to a small volume of crystals, recrystallized with ethanol for 2-3 times, and dried Get withanide.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, in particular to a method for extracting withanolide. Background technique [0002] Indian ginseng is a plant of the genus Withania in the family Solanaceae, and its roots are often used as medicine. Used in Ayurvedic medicine in India for over 3,000 years, it is primarily used as an aphrodisiac, liver tonic, anti-inflammatory and astringent. In recent years, foreign research in this field is more active. Clinical and animal experiments have shown that it can be used to treat bronchitis, asthma, ulcers, weakness, insomnia, and Alzheimer's, anxiety, cognitive and nervous disorders, and Parkinson's disease. According to research, the main component of Indian ginseng root is withanide, which is a steroidal lactone with ergosterane as the skeleton, similar to steroid hormones in the human body, has anti-inflammatory, antibacterial and anti-tumor effects, and can inhibit cancer cells grow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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