The invention discloses a Kinect-based robot self-positioning method. The method includes the following steps that: the RGB image and depth image of an environment are acquired through the Kinect, and the relative motion of a robot is estimated through the information of visual fusion and a physical speedometer, and pose tracking can be realized according to the pose of the robot at a last time point; depth information is converted into three-dimensional point cloud, and a ground surface is extracted from the point cloud, and the height and pitch angle of the Kinect relative to the ground surface are automatically calibrated according to the ground surface, so that the three-dimensional point cloud can be projected to the ground surface, and therefore, two-dimensional point cloud similar to laser data can be obtained, and the two-dimensional point cloud is matched with pre-constructed environment raster map, and thus, accumulated errors in a robot tracking process can be corrected, and the pose of the robot can be estimated accurately. According to the Kinect-based robot self-positioning method of the invention, the Kinect is adopted to replace laser to perform positioning, and therefore, cost is low; image and depth information is fused, so that the method can have high precision; and the method is compatible with a laser map, and the mounting height and pose of the Kinect are not required to be calibrated in advance, and therefore, the method is convenient to use, and requirements for autonomous positioning and navigation of the robot can be satisfied.