The invention relates to a minimal invasive negative pressure bladder puncture fistulation indwelling device and the working method, which pertains to the technical field of medical devices, including a fistulation tube, a fixed board, a negative pressure urine drainage bag and a puncture needle, the fistulation tube is a tube which is provided with an opening at one end and is smoothly closed and bent at the other end, a plurality of side holes are arranged on the inner side wall of the bending part, a spring device is arranged in the negative pressure urine drainage bag, a connection soft tube is arranged at the inlet at the upper end, the end part of the connection soft tube is provided with a connector part, the lower end thereof is provided with an outlet soft tube, switches are arranged on the connection soft tube and the outlet soft tube, the fixed main board body is a broken annular board, the inner side of the annular shape is provided with a serrated protuberance, the broken part of the fixed board is provided with a slidable tooth buckle, and the periphery of the main body board is provided with four forked boards which are evenly distributed. The puncture needle is the needle which takes an appearance of T and is provided with a tiny channel at the inner side that deviates from the center. The working method is that: the local infiltration anesthesia is carried out, the empty needle tries to penetrate for implanting the fistulation tube into the bladder, the fistulation tube is fixed, the negative pressure urine drainage bag and the fistulation tube are connected, and the bladder puncture fistulation is completed.