The invention relates to a method for simultaneous detection of residual quantity of fluvalinate, triadimenol and
coumaphos in
royal jelly. So far, there has not been a method capable of simultaneously detecting residual quantity of fluvalinate, triadimenol and
coumaphos in
royal jelly. The reagents used in the invention comprise chromatographically pure
acetonitrile, water, analytically pure
anhydrous sodium sulfate, n-
propanol, fluvalinate, triadimenol,
coumaphos, analytically pure
ammonia liquor and chromatographically pure
methanol; the method comprises the processes of preparation of a standard
stock solution, preparation of blended standard intermediate liquid, preparation of standard liquid, preparation of a
standard curve, treatment of samples and detection of samples. The invention has the characteristics of simple operation, high detection efficiency, high detection precision, with detection limits for coumaphos, triadimenol and fluvalinate respectively being 1.6 mu g / kg, 2.1 mu g / kg and 2.0 mu g / kg by using the method of determining corresponding concentration on the basis of the equation of
signal to
noise ratio that S / N=3, and capacity of simultaneous detection of residual quantity of fluvalinate, triadimenol and coumaphos in
royal jelly.