Simultaneous detection of fluvalinate, triadimenol and muscarin residues in royal jelly

Abstract

The invention relates to a method for simultaneous detection of residual quantity of fluvalinate, triadimenol and coumaphos in royal jelly. So far, there has not been a method capable of simultaneously detecting residual quantity of fluvalinate, triadimenol and coumaphos in royal jelly. The reagents used in the invention comprise chromatographically pure acetonitrile, water, analytically pure anhydrous sodium sulfate, n-propanol, fluvalinate, triadimenol, coumaphos, analytically pure ammonia liquor and chromatographically pure methanol; the method comprises the processes of preparation of a standard stock solution, preparation of blended standard intermediate liquid, preparation of standard liquid, preparation of a standard curve, treatment of samples and detection of samples. The invention has the characteristics of simple operation, high detection efficiency, high detection precision, with detection limits for coumaphos, triadimenol and fluvalinate respectively being 1.6 mu g / kg, 2.1 mu g / kg and 2.0 mu g / kg by using the method of determining corresponding concentration on the basis of the equation of signal to noise ratio that S / N=3, and capacity of simultaneous detection of residual quantity of fluvalinate, triadimenol and coumaphos in royal jelly.

Description

technical field [0001] The present invention relates to a method for detecting the residues of fluvalinate, triadimenol and amastrophos in royal jelly, in particular to a method for simultaneously detecting the residues of fluvalinate, triadimenol and amastrophos in royal jelly , belongs to a detection method of royal jelly. Background technique [0002] In the production process of royal jelly, it is necessary to detect the residues of fluvalinate, triadimenol and muscarin in royal jelly. The method of residual quantity is usually more complicated, the detection efficiency is low, the detection accuracy is poor, and it is difficult to meet the requirements of the importing country for the residue limit of bee products, which hinders the export of bee products. For example, the publication date is February 04, 2009, and the publication number is CN101358953 Chinese patent, which discloses a method for simultaneous detection of multiple types of agricultural and veterinary d...

AI Technical Summary

Problems solved by technology

[0002] In the production process of royal jelly, it is necessary to detect the residues of fluvalinate, triadimenol and muscarin in royal jelly. The method of residual amount is usually complicated, the detection efficiency is low, and the detection accuracy is poor. It is difficult to meet the requirements of the importing country for the residue limit of bee products, which hinders the export of bee products

For example, the publication date is February 04, 2009, and the publication number is CN101358953 Chinese patent, which discloses a method for simultaneous detection of multiple types of agricultural and veterinary drug residues in bee products. Perchloric acid and extract solution acetate, phosphate or borate solution, control the pH value of 4.5-9.0; centrifuge, extract the filtrate into a solid phase extraction column, elute and dry the extraction column, wash with oxalic acid-methanol solution Column, the eluent is constant volume with methanol aqueous solution, and put into liquid chromatography-tandem mass spectrometry analysis test, the obtained chromatographic peaks are compared with the standard chromatographic peaks of each known drug, and the detected drug is determined according to the retention time and the abundance of mass spectrometry ions The specific name of the method; after the sample is pretreated once, the method can simultaneously extract sulfonamides, quinolones, macrolides, lincosamides, nitroimidazoles, β-lactams, tetracyclines, and chloramphenicol The drug residues of more than 60 veterinary drugs in 11 categories, such as steroids, sulfa synergists, chlordimeform, and triadimenol, can reduce the detection cost. However, due to the excessive amount of components detected by this method, the detection The accuracy is greatly reduced, and it is difficult to effectively achieve the purpose of detecting the residual amount of triadimenol

[0003] At present, there is no method that can simultaneously detect the residues of fluvalinate, triadimenol and muscarinin in royal jelly, which greatly reduces the detection efficiency and increases the detection cost

Images