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Production process of liposome-coated lactase

A production process and lactase technology, applied in the field of lactase, can solve the problems of lactase sensitivity to environmental conditions, easy oxidative deterioration, application defects, etc., and achieve the effects of easy acquisition, avoiding instability, and prolonging the activity cycle.

Pending Publication Date: 2022-03-22
山东源科生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because lactase is sensitive to environmental conditions, it is easy to oxidize and deteriorate, causing defects in application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A production process for liposome-encapsulated lactase, comprising the following process steps:

[0030] (1) Dissolve the membrane material in an organic solvent, remove the organic solvent by evaporation under reduced pressure, and form a lipid film;

[0031] (2) dissolving lactase in a solvent to obtain a lactase solution;

[0032] (3) Add lactase solution to the lipid film, dissolve the lipid film, and sonicate in an ice bath to obtain a liposome-lactase solution;

[0033] (4) Filtrate with a microporous membrane to obtain liposomes encapsulating lactase.

[0034] In step (1), the membrane material includes cholesterol and lecithin, and the weight ratio of cholesterol and lecithin is 1:2. The concentration of the film material in the organic solvent is 3.0mg / mL; the temperature of the vacuum evaporation is 50°C. The organic solvent includes a combination of absolute ethanol and absolute ether.

[0035] In step (2), the solvent is a phosphate buffer solution with ...

Embodiment 2

[0040] A production process for liposome-encapsulated lactase, comprising the following process steps:

[0041] (1) Dissolve the membrane material in an organic solvent, remove the organic solvent by evaporation under reduced pressure, and form a lipid film;

[0042] (2) dissolving lactase in a solvent to obtain a lactase solution;

[0043] (3) Add lactase solution to the lipid film, dissolve the lipid film, and sonicate in an ice bath to obtain a liposome-lactase solution;

[0044] (4) Filtrate with a microporous membrane to obtain liposomes encapsulating lactase.

[0045] In step (1), the membrane material includes cholesterol and lecithin, and the weight ratio of cholesterol and lecithin is 1:6. The concentration of the film material in the organic solvent is 6mg / mL; the temperature of the vacuum evaporation is 70°C.

[0046] In step (1), the organic solvent includes a combination of ethanol and chloroform.

[0047]In step (2), the solvent is a phosphate buffer solution...

Embodiment 3

[0051] A production process for liposome-encapsulated lactase, comprising the following process steps:

[0052] (1) Dissolve the membrane material in an organic solvent, remove the organic solvent by evaporation under reduced pressure, and form a lipid film;

[0053] (2) dissolving lactase in a solvent to obtain a lactase solution;

[0054] (3) Add lactase solution to the lipid film, dissolve the lipid film, and sonicate in an ice bath to obtain a liposome-lactase solution;

[0055] (4) Filtrate with a microporous membrane to obtain liposomes encapsulating lactase.

[0056] In step (1), the membrane material includes cholesterol and lecithin, and the weight ratio of cholesterol and lecithin is 1:4. The concentration of the film material in the organic solvent is 6mg / mL; the temperature of the vacuum evaporation is 60°C.

[0057] The organic solvent includes a combination of ethanol and chloroform.

[0058] In step (2), the solvent is a phosphate buffer solution with a pH v...

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Abstract

The invention belongs to the technical field of lactase, and particularly relates to a production process of liposome-coated lactase, which comprises the following process steps: dissolving a membrane material in an organic solvent, and removing the organic solvent through reduced pressure evaporation to form a lipid membrane; dissolving lactase in a solvent to obtain a lactase solution; adding a lactase solution into the lipid film, dissolving the lipid film, and carrying out ice-bath ultrasonic treatment to obtain a liposome-lactase solution; and filtering by using a microfiltration membrane to obtain the lactase-coated lipidosome. The lactase is wrapped by the lipidosome, the lactase is separated from the external environment, the lactase is protected from being affected by environmental conditions, oxidative deterioration of the lactase is prevented, the activity time of the lactase is prolonged, the stability of the lactase is improved, and the lipidosome can transfer protease into cells through endocytosis or membrane fusion and the like. The problem that the biomacromolecule is difficult to penetrate through a cell membrane can be effectively solved, and the action effect and the utilization rate can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of lactase, in particular to a production process of liposome-encapsulated lactase. Background technique [0002] Lactase, also known as β-galactosidase, can catalyze the hydrolysis of β-D-galactoside and α-L-arabinoside. Lactase breaks down excess lactose in the body into glucose and galactose. Glucose is the energy source for the metabolism of various parts of the human body, and galactose is the structural sugar necessary for the metabolism of the human brain and mucous membrane tissue. It is a necessary tissue for infant brain development and is closely related to the rapid growth of the infant brain. Furthermore, lactase can also generate oligosaccharides through transglycosidation in the human body. These oligosaccharides are a kind of low molecular weight, non-viscous water-soluble dietary fiber#It is only used as a proliferation factor in the intestinal tract by bifidobacteria Utilize # but will no...

Claims

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Application Information

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IPC IPC(8): A61K38/47A61K9/127A61P1/00A61P31/04A61P1/10A61P1/12A61P39/06A61P35/00A61P29/00A61P7/02A61P3/10A61P3/04A61P27/02A61K35/747A61K31/7048A61K31/702
CPCA61K38/47A61K9/1277A61K35/747A61K31/7048A61K31/702A61P1/00A61P31/04A61P1/10A61P1/12A61P39/06A61P35/00A61P29/00A61P7/02A61P3/10A61P3/04A61P27/02C12Y302/01023A61K2300/00
Inventor 孙波
Owner 山东源科生物科技股份有限公司
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