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Escherichia coli capable of producing enterotoxins for piglets and application of escherichia coli

A technology of Escherichia coli and enterotoxin, applied in bacteria, antibacterial drugs, medical preparations containing active ingredients, etc., can solve the problems of difficulty in vaccines, reduction of effective drugs, frequent use of antibiotics, etc., and achieve fast proliferation and proliferation titer. high effect

Active Publication Date: 2022-03-01
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex serotypes of Escherichia coli, it is difficult to control it by vaccines, and antibiotics are frequently used
The long-term use of antibacterial drugs has led to the continuous increase of drug-resistant strains, the continuous expansion of the drug-resistant spectrum, and the reduction of effective drugs, making it difficult to control the disease after the onset and causing great losses to the aquaculture industry.

Method used

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  • Escherichia coli capable of producing enterotoxins for piglets and application of escherichia coli
  • Escherichia coli capable of producing enterotoxins for piglets and application of escherichia coli
  • Escherichia coli capable of producing enterotoxins for piglets and application of escherichia coli

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation of piglet enterotoxigenic Escherichia coli

[0029] 1. Collection and processing of intestinal tissue

[0030] Porcine intestinal tissue was collected, shredded, added 10 times the volume of DMEM medium for grinding, and set aside.

[0031] 2. Pathogen detection in intestinal tissue

[0032] Take 200 μl of the supernatant of the above grinding solution, and use the DNA / RNA extraction kit of Beijing Quanshijin Biotechnology Co., Ltd. to extract DNA according to the instructions. Using the extracted DNA as a template, referring to the detection primers of the Escherichia coli PCR detection method (DB21 / T 2734.1-2017), PCR detection was performed with full gold 2×EasyTaq PCR SuperMix, and positive and negative controls were set at the same time. The test results showed that the grinding liquid of the disease material was positive for Escherichia coli and negative for other pathogens.

[0033] 3. Isolation of E. coli

[0034] Take the grinding liquid...

Embodiment 2

[0051] Example 2 Drug resistance experiment of piglet enterotoxigenic Escherichia coli ETEC-GS2020 CGMCC No.23598

[0052] MacConkey culture medium was purchased from Beijing Aoboxing Biotechnology Co., Ltd., drug-sensitive disc amikacin (amikacin), nitrofurantoin, cephalothin, ceftriaxone, ceftazidime, azithromycin, cefixime, chlorine cefuroxime, ofloxacin, penicillin, streptomycin, cefazolin, ciprofloxacin, norfloxacin, gentamicin, clarithromycin, vancomycin, rifampicin, multi Colistin B, enrofloxacin, erythromycin, and kanamycin were all purchased from Wenzhou Kangtai Biotechnology Co., Ltd.

[0053] Judgment basis: Incubate at 37°C for 18-24 hours, measure the diameter of the inhibition zone. Inhibition zone 15.1mm≤diameter≤20.1mm is highly sensitive, 10.1mm≤diameter<15.1mm is moderately sensitive, and diameter<10.1mm is resistant.

[0054] The results of the drug sensitivity test are shown in Table 2. The strain ETEC-GS2020 CGMCC No.23598 is hypersensitive to cephalothi...

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Abstract

The invention discloses enterotoxigenic escherichia coli for piglets and application of the enterotoxigenic escherichia coli. The enterotoxigenic Escherichia coli of the pig is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the enterotoxigenic Escherichia coli is CGMCC No.23598. The enterotoxigenic Escherichia coli of the pig is the enterotoxigenic Escherichia coli of the pig. The enterotoxigenic Escherichia coli CGMCC No.23598 for the piglets can be used for preventing and treating diarrhea diseases of the piglets, and can be used for preparing biological products for preventing the diarrhea diseases of the piglets and preparing biological products for treating the diarrhea diseases of the piglets.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to piglet enterotoxigenic Escherichia coli and application thereof. Background technique [0002] Escherichia coli disease is one of the more serious infectious diseases that harm newborn or weaned piglets, especially diarrhea and piglet edema in weaned piglets often cause stunted piglets, increased feeding costs, and lead to a large number of deaths in severe cases. Enterotoxigenic Escherichia coli (ETEC) is an important pathogen that causes diarrhea in humans and young animals (newborn piglets, calves, lambs and weaned piglets). morbidity and mortality are high. The Escherichia coli that causes diarrhea in piglets is mainly ETEC, which colonizes the intestinal tract through the binding of its pilus adhesin to the receptors of intestinal mucosal epithelial cells. ETEC can produce one or several enterotoxins, including heat-stable enterotoxin (ST) and heat-labile enterotox...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K45/00A61P1/12A61P31/04C12R1/19
CPCA61K45/00A61P1/12A61P31/04Y02A50/30
Inventor 杨小蓉马良王天恺常昊天
Owner CHINA ANIMAL HUSBANDRY IND
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