Novosphingobium and application thereof in wastewater phosphorus removal

Abstract

The invention relates to Novosphingobium and application thereof in wastewater phosphorus removal. The bacterial strain is Novosphingobium sp. SJB007 and preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.21177. The invention further discloses an application of the Novosphingobium sp. SJB007 in removal of phosphorus in sewage. The application comprises the following steps that (1), strain culture is carried out, specifically, a preservation strain, namely Novosphingobium sp. SJB007, is inoculated into a seed culture medium, culturing is carried out for 24 hours under the conditions of 30 DEG C and 160rpm, centrifuging is carried out to obtain thalli, and the thalli are washed with sterile water to prepare a bacterial suspension with OD600 of 0.50-0.60; and (2), fermenting is carried out to remove phosphorus, specifically, the bacterial suspension prepared in the step (1) is inoculated into phosphorus-containing sewage according to the proportion of 2%, and fermenting is carried out under the conditions that the pH is 4-8 and the temperature is 15-35 DEG C to remove phosphorus. According to the efficient phosphorus-accumulating strain Novosphingobium sp. SJB007, when the concentration of phosphorus in sewage is 10-30mg / L under a proper condition, the removal rate exceeds 97%, and a relatively high removal rate is achieved.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a new sphingobacterium strain and its application in wastewater phosphorus removal. Background technique [0002] The excessive growth of algae caused by eutrophication of the water environment is one of the main problems causing water quality deterioration. Phosphorus is an important factor leading to eutrophication of water bodies, and its pollution sources are mainly domestic, industrial and agricultural wastewater. Therefore, one of the main purposes of wastewater treatment is Phosphorus removal from wastewater. [0003] The traditional physical and chemical phosphorus removal process has low efficiency, complex technology, and easy to cause secondary pollution, while the biological phosphorus removal method has the characteristics of low cost, small workload, high efficiency and wide application range, and is currently the main method for sew...

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Problems solved by technology

[0003] The traditional physical and chemical phosphorus removal process has low efficiency, complex technology, and easy to cause secondary pollution, while the biological phosphorus removal method has the characteristics of low cost, small workload, high efficiency and wide application range, and is currently the main method for sewage phosphorus removal

In biological phosphorus removal, the main role is a type of microorganisms that cause anaerobic phosphorus release, aerobic or anoxic excess phosphorus uptake under the alternating operation of anaerobic / aerobic or anaerobic / anoxic—phosphorus accumulating bacteria ( Phosphorus Accumulating Organisms), the phosphorus content of this type of microorganism is several times higher than that of ordinary bacteria. Current research believes that phosphorus accumulating bacteria can be divided into two types: aerobic phosphorus accumulating bacteria and denitrifying phosphorus accumulating organisms. The reported biological phosphorus removal strains are mainly Including Pseudomonas (Zhu Weiqiang, Chen Shu, Zhang Peiyu. Screening of 2 strains of denitrifying phosphorus accumulating bacteria and their influencing factors. Journal of Environmental Engineering, 2016, 10(4):3295-3302), Enterobacter, Staphylococcus Genus, Paracoccus and Pantoea (Zhang Licheng, Li Yanmei, Yuan Yashu, et al. Screening of 5 strains of phosphorus accumulating bacteria and research on the characteristics of nitrosation and denitrification phosphorus removal. Industrial Water Treatment, 2012, 32(4): 33-35 ), Acinetobacter (Acevedo B, C, Corona J E, et al. The metabolic versatility of PAOs as an opportunity to obtain a highly P-enriched stream for further P-recovery. Chemical Engineering Journal, 2015, 270:459-467), Aeromonas, Pseudomonas Bacillus, Streptococcus, Moraxella and Micrococcus (Wei Ruping, Yan Cheng, Yang Xinyan, et al. Research progress on functional microorganisms to enhance biological phosphorus removal system. Biotechnology Bulletin, 2017, 33(10): 1-8), but most of these strains have disadvantages such as poor tolerance to the natural environment, difficulty in breeding and cultivation, and easy decline in activity. In actual use, the number of viable bacteria is not high, and most of the strains cannot show Good phosphorus removal effect, the phosphorus removal effect needs to be further improved

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