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Nano-micro composite sphere as well as preparation method and application thereof

A composite ball, nano-micro technology, applied in chemical instruments and methods, other chemical processes, alkali metal compounds, etc., can solve the problem of not having to separate individual virus particles or direct separation of trace proteins, and achieve low-cost effects

Active Publication Date: 2021-06-18
XIAMEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Nanospheres are particles with a diameter of nanoscale or micronscale. The nanospheres disclosed in the prior art are mainly used as immune adjuvants or for the purification of proteins and nucleic acids, and do not have the use of separating individual virus particles or direct separation of trace proteins.

Method used

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  • Nano-micro composite sphere as well as preparation method and application thereof
  • Nano-micro composite sphere as well as preparation method and application thereof
  • Nano-micro composite sphere as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This embodiment provides a preparation method of nano-micro composite spheres, specifically as follows:

[0033] 1. Main materials:

[0034] A commercially available 2-inch, 50 μm thick double-sided polished silicon wafer is used as the chip substrate; the diameter of the microsphere is 5 μm, polystyrene material, the inside of the ball contains nano-magnetic particles, and there is no residual magnetism after removing the external magnetic field; the diameter of the nanosphere is 1300nm, two Silicon oxide material, surface amination modification.

[0035] 2. Preparation process:

[0036] (1) Prepare a chip containing a micrometer hole in a tapered hole array;

[0037] The tapered micro-hole chip is prepared by double-sided etching method, and the etching process is as follows: figure 1 shown. A Cr mask layer with a thickness of 300nm was formed on the front of the silicon wafer by ion sputtering, and a layer of Si with a thickness of 300nm was formed on the back of...

Embodiment 2

[0047] This embodiment provides a preparation method of nano-micro composite spheres, specifically as follows:

[0048] 1. Main materials:

[0049] Commercially available porous ceramics are used as the chip substrate, with a thickness of 3mm, an average filter pore size of 0.45-0.8μm, a microsphere diameter of 2μm, polystyrene material, nano-magnetic particles inside the ball, a nanosphere diameter of 100nm, a silicon dioxide material, and a surface Amino modification.

[0050] 2. Preparation process:

[0051] (1) Prepare a chip containing a micrometer hole in a tapered hole array;

[0052] Cylindrical microporous chips were prepared by dry etching, and the etching process was as follows: Figure 4 shown. First, if Figure 4 Shown in (A), on the porous ceramic surface, sputter once dense silicon dioxide thin film, thickness 50nm, adopt spin coating method to coat one deck photoresist evenly on dense silicon dioxide thin film, then as shown Figure 4 As shown in (B), the...

Embodiment 3

[0062] This embodiment provides a preparation method of nano-micro composite spheres, specifically as follows:

[0063] 1. Main materials:

[0064] Commercially available porous ceramics are used as the chip substrate, with a thickness of 3mm, an average filter pore size of 0.45-0.8μm, a microsphere diameter of 2μm, polystyrene material, nano-magnetic particles inside the ball, a nanosphere diameter of 100nm, a silicon dioxide material, and a surface Amino modification.

[0065] 2. Preparation process:

[0066] (1) Prepare a chip containing a micrometer hole in a tapered hole array;

[0067] Cylindrical microporous chips were prepared by dry etching, and the etching process was as follows: Figure 4 shown. First, on the porous ceramic surface, sputter a dense silicon dioxide film with a thickness of 50nm, apply a layer of photoresist evenly on the dense silicon dioxide film by spin coating, and use the photolithography process to make the mask plate The circular hole patt...

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Abstract

The invention discloses a nano-micro composite sphere as well as a preparation method and application thereof. The preparation method has the advantages of high efficiency, rapidness and low cost, the prepared nano-micro composite sphere is formed by embedding a nanosphere in a nanopore in the surface of a micro sphere, the micro sphere and the surface of the nanosphere can be subjected to surface modification according to actual requirements, and magnetic particles are contained in the microsphere. The nano-micro composite sphere can be used for capturing, detecting and separating biological particles or biological molecules.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a nano-micro composite sphere and its preparation method and application. Background technique [0002] Biological cells contain a variety of protein molecules and nucleic acid molecules. In conventional biology, many steps of separation, purification and detection are usually required to obtain target protein molecules or nucleic acid molecules in a large number of biological samples. In conventional biology, single colonies and single cells are mainly obtained through the single colony technology of petri dish and single particle technology of optical micromanipulation, and then the genome is obtained through pure culture and molecular sequencing. However, these biological methods are difficult to obtain single particles of proteins, nucleic acids or viruses, because these individual biomolecules or biological particles are extremely small, belonging to the nanometer or mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30B01L3/00
CPCB01J20/103B01J20/261B01J20/28021B01L3/502707
Inventor 徐方成曹一洲蔡佳达
Owner XIAMEN UNIV
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