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Preparation method of biological membrane for dental implantation

A biofilm and dental implant technology, applied in the field of medical biomaterials, can solve problems such as difficulty in ensuring the stability of biofilm, achieve the effect of flexible texture, increase the speed of vascularization, and increase the gap between collagen fibers

Inactive Publication Date: 2021-06-15
山西奥瑞生物材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the biofilms currently used in dental implants have some problems of immunogenicity or easy degradation, and it is difficult to ensure the stabilization of biofilms

Method used

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  • Preparation method of biological membrane for dental implantation

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Experimental program
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Effect test

Embodiment 1

[0029] see figure 1 ,

[0030] A method for preparing a biofilm for dental implants, comprising the steps of:

[0031] S1, taking skin: take qualified donor skin as raw material, remove the subcutaneous tissue, and take a certain thickness of sliced ​​skin, including the epidermis and part of the dermis;

[0032] S2, removing the epidermis: after cutting the tomographic skin piece obtained in step S1, put it into a sodium chloride solution for treatment, and remove the epidermis to obtain a dermal matrix;

[0033] S3, Ultrasonic to remove cell components: the dermal matrix obtained in step S2 is subjected to ultrasonic treatment in sodium dodecyl sulfate (SDS) solution and trypsin solution, broken and removed cell components;

[0034] S4, virus inactivation: taking the decellularized dermal matrix treated in step S3, soaking in ethanol for virus inactivation;

[0035] S5, freeze-drying: take the decellularized dermal matrix treated in step S4, and perform freeze-drying to o...

Embodiment 2

[0038] see figure 1 , to further elaborate and supplement Example 1, the step S1 is processed by a drum-type dermatologist or an electric dermatologist, and a 0.3-0.6mm sliced ​​skin is taken, and the strict control of the thickness can ensure the stability of the subsequent steps To obtain qualified biofilm materials.

[0039] The concentration of the sodium chloride solution used in the step S2 is 1-2 mol / L, and the tomographic skin is shaken in the sodium chloride solution at a constant temperature of 30-37° C. for 16-36 hours.

[0040] In step S3, when ultrasonically treating the dermal matrix, first put the dermal matrix into 5-20 g / L sodium dodecyl sulfate (SDS) sterile saline solution, and treat it at room temperature under ultrasonic conditions for 0.5-4 hours , break the cells, and wash and remove the cell debris; thus, the cells can be broken and the cytokine sheets can be cleaned easily;

[0041] Then put it into 1-2.5g / L trypsin sterile saline solution, and wash ...

Embodiment 3

[0051] This application prepares a biofilm derived from human tissue, and introduces ultrasonic technology into the preparation process to completely remove cell components. Trypsin can increase the gap between collagen fibers and increase the speed of vascularization after implantation; using unique freeze-drying technology, The prepared biofilm is flexible and easy to use; it overcomes the immunogenicity derived from heterogeneous materials and the easy degradation of synthetic collagen films, and provides an extracellular matrix biomaterial for dental implants.

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Abstract

The invention discloses a preparation method of a biological membrane for dental implantation, which comprises the following steps: taking qualified donor skin as a raw material, removing subcutaneous tissues, and taking a faulted skin graft with a certain thickness; treating the faulted skin graft in a sodium chloride solution, and removing an epidermal layer to obtain a dermal matrix; performing ultrasonic treatment on the dermal matrix in a sodium dodecyl sulfate (SDS) solution and a trypsin solution, crushing and removing cell components; taking the acellular dermal matrix, and soaking the acellular dermal matrix in ethanol to carry out virus inactivation; performing freeze drying to obtain an acellular dermal matrix biological membrane; and packaging the freeze-dried biological membrane, and carrying out irradiation sterilization. According to the invention, an extracellular matrix repairing material can be obtained, the immunogenicity of a heterogeneous material and the degradability of a synthetic collagen membrane are overcome, and an extracellular matrix biological material is provided for dental implantation.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a method for preparing a biofilm for dental implantation. Background technique [0002] In the field of stomatology, dental implants are a common method for repairing missing or defective dentition. The bone mass of the implant site is the key to the success of the implant. If the alveolar ridge is too low at the implant site, bone tissue defects will affect the implant placement effect. [0003] Guided bone regeneration technology uses a biofilm to repair the bone in the bone defect and gingival soft tissue defect area. At present, the repair films used in China include Bio-Gide absorbable biofilm from Switzerland, Haiao oral repair film from Yantai Zhenghai Biotechnology Co., Ltd., and domestic Bot medical collagen repair film. Among them, Bio-Gide and Haiao oral repair membranes are derived from heterogeneous materials, which have a kind of immunogenicity; Bote m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/362A61L27/3633A61L27/3687A61L27/3691A61L27/50
Inventor 马绍英杨婷李晶任晓琦乔玮李靖石浩
Owner 山西奥瑞生物材料有限公司
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