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Therapeutic HPV vaccine based on chimpanzee adenovirus vector as well as preparation method and application of therapeutic HPV vaccine

A therapeutic, adenovirus technology, applied in the field of therapeutic vaccines

Inactive Publication Date: 2020-12-29
IMMUNE PATH BIOTECHNOLOGY SUZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the field of therapeutic vaccines for cervical cancer caused by HPV infection, although the research on adenovirus vaccines has a history of nearly two decades, most of them are still in the preclinical research stage

Method used

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  • Therapeutic HPV vaccine based on chimpanzee adenovirus vector as well as preparation method and application of therapeutic HPV vaccine
  • Therapeutic HPV vaccine based on chimpanzee adenovirus vector as well as preparation method and application of therapeutic HPV vaccine
  • Therapeutic HPV vaccine based on chimpanzee adenovirus vector as well as preparation method and application of therapeutic HPV vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 1. Experimental materials

[0060] 1.1. Cells

[0061] The HEK293 cell line was purchased from the ATCC cell bank in the United States (product number: CRL-1573), and the medium was DMEM (GIBCO, #11995-040) containing 10% fetal bovine serum (GIBCO, #10091-148).

[0062] TC-1 cell line was purchased from ATCC cell bank in the United States (product number: CRL_2785), and the medium was RPMI 1640 (GIBCO, #72400-047) containing 10% fetal bovine serum (GIBCO, #10091-148).

[0063] 1.2. BALB / c mice and C57BL / 6 mice.

[0064] The mice involved in this experiment were all purchased from Shanghai Xipuer-Bikay Experimental Animal Co., Ltd., and all animal experiments were carried out in the SPF barrier of Shanghai Experimental Animal Research Center.

[0065] 2. Experimental method

[0066] 2.1. Packaging of recombinant chimpanzee adenovirus (taking ChAd63-HPV-mu-GMCSF recombinant adenovirus as an example)

[0067] The plasmid pChAd63-HPV-mu-GMCSF was synthesized by Suzhou J...

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Abstract

The invention discloses a therapeutic HPV vaccine based on a chimpanzee adenovirus vector as well as a preparation method and application of the therapeutic HPV vaccine. The therapeutic HPV vaccine isan immunogenic HPV recombinant adenovirus vaccine obtained by packaging and processing HPV virus vaccine vector plasmids, and the HPV virus vaccine vector is a replication-defective chimpanzee adenovirus vector. The HPV therapeutic vaccine is constructed by using the replication-defective chimpanzee adenovirus vector, the influence of the pre-stored immunity of a human adenovirus vector in a human body on the vaccine efficacy is overcome, and a vaccine product which is simple to prepare, low in cost, good in safety and free of adjuvants is expected to be obtained. The therapeutic HPV vaccinehas good immunogenicity and anti-tumor efficacy, can induce high-level antigen-specific T cell reaction and anti-tumor efficacy in a mouse body through single muscle immunization, and is expected to show good humoral and cellular immunotherapy effect can be embodied in human clinical tests.

Description

technical field [0001] The invention relates to the technical field of therapeutic vaccines, in particular to a therapeutic HPV vaccine based on chimpanzee adenovirus vector, its preparation method and application. Background technique [0002] High-risk human papillomavirus (HPV) infection is the cause of almost all invasive cervical cancers and several other anal and genital malignancies. In China, high-risk HPV types 16 and 18 (HPV16 / 18) were positive in more than 70% of cervical intraepithelial neoplasia stage 2 / 3 (CIN2 / 3) and cervical squamous cell carcinoma (SCC) samples (Chen, W., Molijn, A., Enqi, W., et al. (2016). The variable clinicopathological categories and role of human papillomavirus in cervical adenocarcinoma: A hospital based nation-wide multi-center retrospective study across China. Int J Cancer . 139(12), 2687-2697. Chen, W., Zhang, X., Molijn, A., et al. (2009). Humanpapillomavirus type-distribution in cervical cancer in China: the importanceof HPV 16 a...

Claims

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Application Information

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IPC IPC(8): A61K39/12A61P31/20A61P35/00
CPCA61K39/12A61P31/20C12N2710/20034A61K2039/5256C12N15/86C07K14/005C07K14/5434C07K14/535C12N2710/10343C12N2800/107C12N2800/22C12N2710/20022A61K2039/53
Inventor 史力张超刘海涛侯永凡张玲莉李军瑞周晨亮田文莉刘革
Owner IMMUNE PATH BIOTECHNOLOGY SUZHOU CO LTD
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