3D-printing PCL-vancomycin anti-infection bone tissue engineering scaffold, and preparation method and application thereof

Abstract

The invention provides a novel 3D-printing PCL-vancomycin anti-infection bone tissue engineering scaffold, and a preparation method and an application thereof and belongs to bioengineering human implants. In the engineering scaffold, poly-epsilon-caprolactone and vancomycin respectively serve as a base material A and an anti-MRSA medicine B, wherein the A is heated by a heating system in a meltingextrusive shaping 3D printer to form a liquid substance C; then in a FDM extrusion channel, the C is gradually cooled and meanwhile is mixed with a solution, containing the B, at a spinning nozzle ofthe 3D printer, thus extruding shaped fiber bundle D; by means of the jointing structure of different layers and angles of the fiber bundles D, the product is produced through steps of room temperature shaping at 23 DEG C, drying, and ethylene oxide disinfection. The scaffold has simple and reliable structure, controllable appearance and microstructure, reliable mechanical properties and controllability of medicine release performance. The scaffold is convenient to implant and is invasive-less and low-cost.

Description

technical field [0001] The invention relates to a 3D printed PCL-vancomycin anti-infection bone tissue engineering scaffold and its preparation method and application, belonging to a bioengineered human implant, which can be used to treat various pathogenic bacteria, such as: Staphylococcus aureus, Treatment of bone destruction caused by Streptococcus pyogenes, β-hemolytic Streptococcus, Bacillus anthracis, Bacillus diphtheria, etc. Background technique [0002] Infection is defined as a disruption of the balance between the host tissue and the local concentration of microorganisms, generally considered to be greater than 10 5 Each gram of tissue or the presence of pathogenic bacteria such as Staphylococcus aureus, Streptococcus pyogenes, and β-hemolytic streptococcus is considered to be an infection. The purpose of infection treatment is to provide effective anti-infective drugs, adequate blood supply, reduce the bacterial load of the wound to achieve the elimination of lo...

AI Technical Summary

Problems solved by technology

However, these materials all have certain defects, including: 1. HA, CPC and other materials have high brittleness and low processing performance, and it is difficult to process and plasticize a specific three-dimensional shape, which often depends on the three-dimensional configuration of the material itself; 2. PGA, Materials such as PLA have low biological activity, elastic modulus, tensile strength, and three-dimensional finishing methods; Medication characteristics in the infection stage (acute stage, subacute stage, chronic infection) to achieve effective and reasonable drug release; 4. Biologically active factors have the possibility of immune rejection, pathogen transmission, allergic reaction, potential tumorigenicity, and teratogenicity, and biosafety The safety cannot be guaranteed, and clinical-grade rhBMP-2, TGF-β and other biological factors often need to be imported, and the price is expensive, and the purchase price per mg reaches nearly a thousand dollars

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