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Method for restoring arsenic-polluted soil by plant-microorganism combination

A combined remediation and arsenic-contaminated technology, applied in the field of soil remediation, can solve the problems of windmill grass that are rarely reported, and achieve the effects of water and soil conservation, strong environmental adaptability, and vigorous growth

Active Publication Date: 2018-12-28
HECHI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, scholars at home and abroad have done a lot of research on the joint restoration technology of plants and microorganisms polluted by arsenic, and have also obtained rich research results. However, most of the studied plants are concentrated on centipede grass, while the research on windmill grass is rarely reported.

Method used

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  • Method for restoring arsenic-polluted soil by plant-microorganism combination
  • Method for restoring arsenic-polluted soil by plant-microorganism combination
  • Method for restoring arsenic-polluted soil by plant-microorganism combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Plant Screening

[0026] Select the herbaceous plants that grow well in a tailing mining area in Hechi City, Guangxi, ghost needle grass, southern mugwort, centipede grass, windmill grass, quinoa, bitter grass, ageratum, wood hibiscus, brick seedlings, big-leaved buddleia, ramie, For the genus Rushgrass and Plumeria, the roots and soil were dug up together, and 3 plants with similar plant heights were selected as parallel samples for each herb plant; each herb plant was separated from the rhizosphere soil on each herb plant, and each herb plant was obtained separately. Plant materials and various soil samples; air-dry each soil sample in a cool and dry place, pick out stones, put them into a mortar, grind them through a 0.15mm sieve and dry them for later use; clean each herb plant material separately, dry them, and measure The plant height and fresh weight are dried at 50°C for 48 hours, and the dry weights of the roots and aerial parts of each plant material...

Embodiment 2

[0046] Example 2 Microbial Screening

[0047] Under sterile conditions, 10 g of the pretreated soil sample with the highest arsenic concentration in Example 1 was added to 90 mL of sterile water containing glass beads, vortexed and shaken, and 1 mL was added to 9 mL of sterile water. Then dilute to a concentration of 10 -6 、10 -7 、10 -8 Then take 200 μL and spread it on LB solid medium, set up three replicates for each gradient, place them in a 37°C incubator and culture them, and pick single colonies with obviously different shapes to draw lines after the colonies grow out Cultivate and obtain 10 strains of primary screening bacteria with obviously different shapes, which are respectively marked as CP1-CP10, and the arsenic-containing LB medium without bacteria is used as a control, which is recorded as CK; the obtained primary screening bacteria CP1-CP10 are inoculated into LB Cultivate in liquid culture medium at 37°C and 180rpm for 12h, centrifuge at 10,000rpm in a ster...

Embodiment 3

[0053] Embodiment 3 plant-microorganism combined restoration

[0054] First, the microorganism CP3 in Example 2 was inoculated into 100 mL of LB liquid medium, and cultivated for 12 hours to obtain the CP3 bacterial liquid, which was set aside.

[0055] Add 1 kg of uncontaminated soil to the pot, followed by 1 mg·mL -1 arsenic standard solution, so that the concentration of arsenic in the potting soil is 5mg·kg -1 ,, planted the windmill grass with the same growth, and added 50mL prepared nutrient solution, slowed down the seedlings for 7 days, then took 20mL of CP3 bacterial solution and added it to the rhizosphere soil of the windmill grass, with the windmill grass without bacteria as a control, record For CK, each group was repeated three times, and then watered once every two days to maintain water content (the amount of water added was kept at 60% of the field water capacity), and 1 nutrient solution was added after 10 days. Remove, wash, and use 5mmol·L -1 The roots w...

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Abstract

The invention discloses a method for restoring arsenic-polluted soil by plant-microorganism combination. The method comprises the following steps of firstly, screening plants and microorganisms, so asto obtain plant clinopodium urticifolium with high arsenic resistance and microbial staphylococcus saprophyticus with high arsenic resistance; next, planting the plant clinopodium urticifolium with high arsenic resistance into heavy metal arsenic-polluted soil, and adding the arsenic-resistant microbial staphylococcus saprophyticus into the root soil of arsenic-enriched plant; and carrying out conventional culture, watering and moisturizing, wherein arsenic in the enriched soil is restored by utilizing the absorption effect of the arsenic-enriched plants on the arsenic and the strengthening effect of the arsenic-resistant microorganisms, and the aim of repairing the arsenic-polluted soil in a tail mining area can be achieved by repeating the above process. The soil restoring method is good in effect, low in cost and easy to manage and operate, no secondary pollution is generated, and the method has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of soil remediation, and in particular relates to a method for jointly remediating arsenic-contaminated soil by using plants and microbes. Background technique [0002] The problem of soil heavy metal pollution is an environmental issue of global concern. According to statistics, the total rate of heavy metals in the country's soil exceeds the standard is 16.1%, mainly due to the pollution of six heavy metals: cadmium, nickel, copper, arsenic, mercury, and lead. Among them, arsenic is a toxic and carcinogenic chemical element, which is widely distributed. The main source of arsenic in the soil is agriculture and industry, which is extremely harmful to the environment, animals and plants, and can even enter the human body through the food chain to cause chronic poisoning of the human body. , so arsenic pollution has become a global environmental problem. Soil is an important part of the human living environm...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10
CPCB09C1/10B09C1/105
Inventor 李秀玲辛磊韦岩松覃拥灵覃勇荣覃国乐谢彦军许仁智
Owner HECHI UNIV
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