Reagent for liquefying phlegm and protecting nucleic acid

Abstract

The invention provides a reagent for simultaneously liquefying phlegm and effectively protecting nucleic acid. The reagent contains guanidine, a cysteine derivative and a macromolecule inert particle,wherein guanidine is one or more of guanidine hydrochloride, guanidinium isothiocyanate, guanidine sulfate and guanidine carbonate; the cysteine derivative is one or more of cysteine, acetylcysteine,homocysteine, methylcysteine and cysteine hydrochloride; and the macromolecule inert particle is one or more of a polypropylene particle, a polyethylene particle, a polyvinyl chloride particle and apolystyrene particle. The reagent is a mixed solid of powder and particles and is capable of well permeating into a phlegm sample, and the phlegm sample is adequately mixed, so that phlegm can be adequately liquefied; and meanwhile, the reagent is capable of protecting nucleic acid in the phlegm sample from being degraded at a normal temperature for a long time. The reagent contains simple components and is low in cost, and the production process is simple, and the reagent is easy to use and operate and very suitable for wide clinical use.

Description

technical field [0001] The method belongs to the technical field of sample processing and preservation for medical testing, and relates to a sputum liquefaction and nucleic acid protection reagent. The technical key lies in promoting sample liquefaction and protecting nucleic acid in the sample from degradation. Background technique [0002] Phlegm is the secretion of the human respiratory tract. It is pushed from the lungs to the respiratory tract through the movement of bronchial cilia and epithelial cilia. Finally, it is coughed out from the trachea through the normal cough reflex of a person. Normal people have very little sputum. A small amount of mucus secreted by moist airways. When people inhale harmful microorganisms such as irritating gas, dust, pathogenic bacteria, and viruses, inflammation may occur in the upper respiratory tract; or if lung diseases occur, such as bronchiectasis, lung abscess, lung cancer, etc., the secretion of the respiratory tract will increa...

AI Technical Summary

Problems solved by technology

[0007] The sputum sample has a high viscosity, conventional liquefaction treatment takes a long time and the sample is not thoroughly mixed. At the same time, the nucleic acid in the sputum sample is easily degraded. At present, there is a lack of low-cost, simple production process and operation, and can fully promote sample mixing and at room temperature. A method for effectively protecting sample nucleic acid under certain conditions, the invention provides a reagent for effectively liquefying sputum and protecting nucleic acid in sputum samples

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