A kind of synchronous extraction method of total triterpenes and polysaccharides of Antrodia antrodia

Abstract

The invention discloses a method for synchronously extracting total triterpenes and polysaccharides of Antrodia camphorata. The method comprises the following steps: taking fruit body powder of Antrodia camphorata, adding an alkaline grinding aid for ball milling to obtain the ball-milled powder; adding the ball-milled powder to In distilled water, at a constant temperature of 30-70°C, carry out a high-speed shear reaction at 5000-10000rmp for 5-20min, and centrifuge to obtain a supernatant; adjust the pH of the supernatant to acidity, stir evenly, and place it in a 4°C refrigerator to stand until After the precipitation is complete, filter to obtain filtrate and precipitate. The filtrate is concentrated and dried to obtain total polysaccharide extract, and the precipitate is naturally dried to obtain total triterpenoid extract. The extraction rate of the total triterpenes of Antrodia camphorata is as high as 13.72%, the extraction rate is increased by nearly 2 times, the extraction rate of polysaccharides is as high as 12.94%, and the extraction rate is increased by nearly 1 times.

Description

[0001] (1) Technical field [0002] The invention relates to an extraction method of effective components of fungi, in particular to a combined extraction method of total triterpenoids and polysaccharides of Antrodia camphorata. [0003] (2) Background technology [0004] Antrodia Cinnamomea is a unique wood-rot fungus in Taiwan. Studies have found that Antrodia Cinnamomea contains a variety of physiologically active components, the most important two being triterpenes and polysaccharides. There are more than 200 kinds of total triterpenes of Antrodia camphorata, which have anti-cancer, anti-inflammatory, anti-virus and liver protection effects. Antrodia camphorata polysaccharides have anti-cancer, immunity-enhancing, and anti-oxidation effects. [0005] Total triterpenoids of Antrodia camphorata are easily soluble in organic solvents, and polysaccharides are easily soluble in water. At present, most of the traditional methods for preparing triterpenes and polysaccharides use ...

AI Technical Summary

Problems solved by technology

The extraction time of this method is 6-12h, which is too long

[0007] (2) Lu Zhenming (Lu Zhenming, Research on deep liquid fermentation of Antrodia camphorata and its triterpenoids, doctoral dissertation of Jiangnan University) compared the yields of triterpenoids extracted by different organic solvents, and finally determined the best extraction process of Antrodia camphorata triterpenoids The conditions are: ethanol concentration 86%, extraction temperature 75°C, liquid-solid ratio 37:1, extraction 2 times, under these conditions the theoretical maximum yield of Antrodia camphorata triterpenoids is 3.23%, the extraction time is long, the use of organic solvents and the extraction rate Low

[0008] (3) Chinese patent CN104479044 extracts polysaccharides from Antrodia cinnamomea, extracts them under reflux with ethanol for 3 times, extracts residues with hot water at 60° C. for 1 hour, and the extraction rate of Antrodia camphorata polysaccharides is 3.93%. This method requires a large amount of ethanol consumption, and the operation is complicated. Long extraction time and low polysaccharide extraction rate

Research on the Extraction Conditions of Intracellular Polysaccharides from Antrodia Camphorata Mycelium, Food Research and Development, 2008, 10, 29(10): 17-19), the extraction process of polysaccharides from Antrodia camphorata mycelium was studied by using the secondary general rotation combination design method The optimal combination of conditions, when the extraction time is 4.7h, the liquid-solid ratio is 46 times, and the extraction temperature is 76.8°C, the theoretical value of the polysaccharide extraction rate in the mycelia of Antrodia camphorata is 8.8%. Low

[0010] (5) CN104193843, the optimal parameters for the extraction of intracellular polysaccharides from Antrodia camphorata mycelium, when the extraction time is 4.7h and the extraction temperature is about 75°C, the theoretical value of the extraction rate is 8.8%. There is also a long extraction time and a low extraction rate The problem

[0011] (6) Liu Hua et al. (Liu Hua, Zhou Kun, etc., Research on the Extraction Technology and Activity of Polysaccharides from the Mycelium of Antrodia Camphorata, Food Science, 2007, 28(06): 243-245) optimized the mycelium of Antrodia camphorata For the extraction process of body crude polysaccharides, the best conditions are solid-liquid ratio 1:30, temperature 90°C, time 2 hours, polysaccharide extraction rate 10%, this method has high extraction temperature, and the extraction rate of polysaccharides is relatively improved, but high temperature is easy to destroy the activity of polysaccharides

[0012] (7) Chinese patent CN103655641 carries out hot water leaching and ethanol leaching to Antrodia camphorata successively, and Antrodia camphorata polysaccharides and triterpenoids are extracted to a certain extent, but the time required for step-by-step leaching is long, and no extraction is given. Rate

[0014] (1) Guo Danzhao et al. (Guo Danzhao, Zheng Wei et al. Ultrasonic-assisted extraction of active substances from Antrodia camphorata mycelium, Food and Machinery, 2016, 1(32): 158-161) Using the mycelium of Antrodia camphorata as raw material, ultrasonic The extraction rate of total triterpenoids and polysaccharides of Antrodia antrodia was 3.8% and 2.63% respectively. Compared with the traditional extraction method, the extraction time was shorter, but the extraction rate was not significantly improved.

[0015] (2) Wang Gong et al. (Wang Gong, Wang Jin, Xu Wei. Research on Extraction Technology of Total Triterpenes of Antrodia Antrodia, Modern Chinese Medicine Research and Practice, 2011, 25(6):67-68) Using the fruiting bodies of Antrodia Cinnamomea as raw materials, they explored the ultrasonic extraction method Optimum process conditions, the extraction rate of triterpenes is 10.2% under the optimal conditions, and the ultrasonic solvent of this method is ethanol, which is not environmentally friendly

[0016] (3) Chinese patent CN105998091, under ultrasonic conditions, use ethanol extraction-water precipitation to extract triterpenoids in Antrodia camphorata, this method is simple to operate, but still uses organic solvents such as ethanol, which is not environmentally friendly, and the extraction rate is not given

Supercritical CO 2 Although the extraction reduces the pollution of organic solvents, the extraction time is as long as 4 hours, and the number of ultrasonic extractions is 3 times, each time 15 minutes. The total extraction time is long, and the extraction rate is not given. The operation is complicated and the cost is high.

[0023] (2) Chinese patent CN104987429, using enzymatic hydrolysis, supercritical extraction, ion exchange chromatography, membrane separation ultrafiltration and chromatographic purification technology to achieve the separation and extraction of Antrodia camphorata compound components, enzymatic hydrolysis to break the cell wall, a relative increase of three The dissolution rate of terpenes and polysaccharides, but this method does not give the extraction rate, and the separation and purification process of triterpenes and polysaccharides is complicated to operate, and the operation cost of enzymatic hydrolysis is higher, so the value of its application needs to be further demonstrated

[0025] (1) Chinese patent CN1290554, using mechanochemistry combined with supercritical CO 2 Extract the effective active ingredients in Ganoderma lucidum, the extracted effective active ingredients account for 37%-37.6% of the Ganoderma lucidum spore powder, but this method is aimed at mechanically destroying the cell wall or combined with enzymatic hydrolysis to break the cell wall and increase the dissolution rate of active ingredients. The extraction of polysaccharides and polysaccharides is not targeted, and the composition and extraction rate of active ingredients are not mentioned. The extract components are complex, and supercritical CO 2 The organic solvent ethanol is still used as the entrainer in the extraction, which is not environmentally friendly

[0026] (2) Chinese patent CN104231105, using constant temperature high-speed shearing method combined with enzymatic hydrolysis to extract sea anemone polysaccharides, constant temperature high-speed shearing method to crush raw materials, the yield of sea anemone polysaccharides is improved, but this method, enzymatic hydrolysis cost is higher, constant temperature high-speed shearing After cutting, extract with water for 2-4 hours, the extraction time is long and the efficiency is low

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