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Composite probiotic lactobacillus powder as well as preparation method and application thereof

A lactic acid bacteria powder and probiotic technology, applied in the biological field, can solve problems such as increased content of Bacteroidetes, different strains and contents of probiotics, and reduced diversity of intestinal flora, so as to protect nasal cavity infection and improve the body's immunity , Improve the effect of gastrointestinal symptoms

Active Publication Date: 2018-05-08
BEIJING SCITOP BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, studies have found that the intestinal flora of patients with irritable bowel syndrome has deviated, and the ratio of Firmicutes to Bacteroidetes has increased, including Ruminococcus, Clostridium, Proteus Proteobacteria, Escherichia Coli, Enterococcus, Enterobacter, Prevotella, Dorea, etc. Sex is reduced, in which the number of beneficial bacteria such as Bifidobacterium, Lactobacillus, Bacteroides and Faecalibacterium decreases
From the perspective of regulating intestinal flora, probiotic therapy has made significant progress in recent years, but the curative effect varies due to the different strains and contents of probiotics

Method used

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  • Composite probiotic lactobacillus powder as well as preparation method and application thereof
  • Composite probiotic lactobacillus powder as well as preparation method and application thereof
  • Composite probiotic lactobacillus powder as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Blueberry-flavored composite probiotic lactic acid bacteria powder

[0035] A method for preparing Lactobacillus plantarum C2 bacterial powder, Lactobacillus casei Zhang bacterial powder, Lactobacillus plantarum P-8 bacterial powder, and Bifidobacterium animalis V9 bacterial powder includes the following steps:

[0036] (1) The fermentation strains of Lactobacillus plantarum C2, Lactobacillus casei Zhang, Lactobacillus plantarum P-8, and Bifidobacterium animalis V94 were separately fermented at high density: a loop of activated Lactobacillus plantarum C2 and cheese The slant cells of Lactobacillus Zhang, Lactobacillus plantarum P-8, and Bifidobacterium animalis V9 were respectively inoculated into MRS medium and cultured at the same temperature of 33-37℃ and the same speed of 50-100rpm for 18-24h, Obtain the first-level seed liquid;

[0037] (2) Transfer the cultured first-level seed solution to the MRS medium at an inoculum amount of 3%-10% (v / v) for secondary acti...

Embodiment 2

[0048] Example 2 Preparation of strawberry flavor composite probiotic lactic acid bacteria powder

[0049] Components by weight: 6 grams of Lactobacillus casei Zhang powder, 5 grams of Bifidobacterium animalis V9 powder, 4 grams of Lactobacillus plantarum P-8 powder, 5 grams of Lactobacillus plantarum C2 powder, Fructooligosaccharides 50 grams, 30 grams of strawberry powder;

[0050] Select Lactobacillus casei Zhang bacterial powder, animal Bifidobacterium V9 bacterial powder, Lactobacillus plantarum P8 bacterial powder and Lactobacillus plantarum C2 bacterial powder according to the requirement of the number of live bacteria: the number of live bacteria of Lactobacillus casei Zhang is 5.0×10 9 CFU / g, the number of live Bifidobacterium animalis V9 6.0×10 9 CFU / g, the number of Lactobacillus plantarum P-8 viable bacteria 3.0×10 9 CFU / g, the number of Lactobacillus plantarum C2 viable bacteria 2.0×10 9 CFU / g;

[0051] Pre-mix the bacterial powder in parts by weight;

[0052] Select the ...

Embodiment 7

[0056] Example 7 Effect of compound probiotic lactic acid bacteria powder on the treatment of irritable bowel syndrome

[0057] 1. Research object

[0058] A selection of 120 patients with IBS who met the Rome III diagnostic criteria in the outpatient and inpatient departments of the Gastroenterology Department of a hospital were selected as the research objects, aged 18-80 years old, regardless of gender. All patients had completed hematuria and stool routine, liver and kidney function, abdominal ultrasound, chest film, gastrointestinal endoscopy, and capsule endoscopy, and no obvious organic changes were found. Exclusion criteria: accompanied by other gastrointestinal diseases, heart, liver and kidney dysfunction, malignant tumors, diabetes, persons with a history of abdominal surgery, pregnant or breastfeeding women, taking drugs that affect the observation of this test (antibiotics, Chinese medicine, etc.), autoimmune diseases, and those who cannot be followed up on schedule. ...

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PUM

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Abstract

The invention provides composite probiotic lactobacillus powder as well as a preparation method and application thereof. The composite probiotic lactobacillus powder is prepared by compounding lactobacillus casei Zhang powder, bifidobacterium animalis V9 powder, lactobacillus plantarum P-8 powder, lactobacillus plantarum C2 powder, prebiotics and fruit and vegetable powder. The composite probioticlactobacillus powder has the remarkable protective effect on irritable bowel syndrome, oral infection and nasal infection, has the remarkable prevention effect on occurrence of upper respiratory infection, can improve gastrointestinal symptoms and adjust intestinal flora, and can improve the immunity of the organism.

Description

Technical field [0001] The invention belongs to the field of biological technology, and specifically relates to a composite probiotic lactic acid bacteria powder and a preparation method and application thereof. Background technique [0002] The intestine is the largest digestive organ of the human body, providing an important role for the human body to digest food and absorb nutrients. In the past 15 years, with the rapid development of high-volume sequencing technology and in-depth research on the intestinal tract, the term intestinal flora has appeared more and more frequently in people's sight. The intestinal flora has been regarded as another important organ of the human body. The human intestinal flora adheres and grows in the nutrient-rich intestine, metabolizes food for the host, provides energy and nutrients, and inhibits the growth of pathogenic microorganisms. The intestinal flora has been formed after the host is born, and the intestinal flora has participated in th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61K36/88A61K36/87A61K36/815A61K36/736A61K36/732A61K36/73A61K36/725A61K36/45A61K36/42A61K36/33A61K36/23A61K36/22A61K36/185A61K9/14A61P1/00A61P11/00A61P1/02A61P11/02A61P37/04C12R1/25C12R1/01C12R1/245A61K35/745A61K31/716A61K31/702A61K31/733
CPCA61K9/14A61K31/702A61K31/716A61K31/733A61K35/745A61K35/747A61K36/185A61K36/22A61K36/23A61K36/33A61K36/42A61K36/45A61K36/725A61K36/73A61K36/732A61K36/736A61K36/815A61K36/87A61K36/88C12N1/20A61K2300/00A61K9/0095A61P31/00A61P1/00A61P11/00
Inventor 张建军马晨王记成
Owner BEIJING SCITOP BIO TECH CO LTD
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