A kind of abnormal saccharophila Aeromonas and its application

A single cell bacteria, sugar gas technology, applied in the direction of bacteria, biological water/sewage treatment, sustainable biological treatment, etc., can solve the problems of secondary pollution, ineffective reproduction, high operating costs, etc., to achieve the effect of ensuring COD

Active Publication Date: 2020-02-18
ZHEJIANG SHUANGLIANG SUNDA ENVIRONMENTAL PROTECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is far from enough to rely solely on physical and chemical technologies to treat catering wastewater. There are shortcomings such as limited processing capacity, long treatment operation cycle, high operating costs, and easy to cause secondary pollution. The biotechnology method is used to treat catering wastewater because microorganisms use catering As a carbon source and energy source for normal growth, waste oil is hydrolyzed into glycerol and fatty acids under the action of lipase and other enzymes, and finally decomposed into C0 2 、H 2 0.CH 4 , has the advantages of safety, non-toxicity, high efficiency, low cost, no secondary pollution and easy reproduction and domestication
[0003] However, in ordinary sewage treatment equipment, there is no or low content of grease-degrading bacteria, which cannot effectively multiply into dominant bacteria, resulting in insufficient decomposed lipase, and a large amount of grease is discharged without being decomposed, and the COD of the effluent seriously exceeds the standard.

Method used

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  • A kind of abnormal saccharophila Aeromonas and its application
  • A kind of abnormal saccharophila Aeromonas and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Screening of oil-degrading bacteria

[0025] 1. Separation and purification: Take oily sewage samples from the sewage well of Lixiyuan canteen of Jiangnan University, add LB liquid medium to prepare a 20wt% suspension, then dilute it to an appropriate concentration and spread it on the LB solid medium plate Incubate at 37°C for 24h.

[0026] 2. Primary screening: Use an inoculation needle to select a single colony point and transfer it to the screening medium plate, culture at 37°C for 24 hours, select the strain that produces a transparent circle, insert it into 50ml LB liquid medium, and cultivate it on a shaker at 37°C and 200rpm for 8 hours. Take the cultured bacterial solution and dilute it to an appropriate concentration and spread it on an LB plate. If a single colony still produces a transparent circle, it will be used as the primary screening strain, such as figure 1 shown.

[0027] 3. Rescreening: insert the bacterium solution of the above-mention...

Embodiment 2

[0033] Embodiment 2: identification of strain

[0034] Using 16Sr DNA analysis, the oil-degrading bacteria were identified as: Aeromonas genus, Aeromonas abnormal saccharophila; scientific name Aeromonas allosaccarophila.

[0035] Extract bacterial genomic DNA, design primers 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' (SEQ ID No.1), 1492R: 5'-GGTTACCTTGTTACGACTT-3' (SEQ ID No.2), RCR conditions: 94°C pre-denaturation for 5 minutes, Denaturation at 94°C for 30s, annealing at 55°C for 30s, extension at 72°C for 2min, 30 cycles, extension at 72°C for 10min, and incubation at 12°C.

[0036] The sequencing result is SEQ ID No.3:

[0037] AAGGTTAAGCTATCTACTTCTGGTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCA

[0038] CCGCAACATTCTGATTTGCGATTACTAGCGATTCCGACTTCACGGAGTCGAGTTGCAGACTCCGATCCGGACTACGACGC

[0039] GCTTTTTGGGATTCGCTCACTATCGCTAGCTTGCAGCCCTCTGTACGCGCCATTGTAGCACGTGTGTAGCCCTGGCCGTA

[0040] AGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTATCCCGGCAGTCTCCCTTGAGTTCCCCACCATTACGT

[00...

Embodiment 3

[0055] Embodiment 3: the preparation of bacterial agent

[0056] Aeromonas saccharophila CY01 was inoculated into liquid LB medium, cultured on a shaker at 37°C and 200 rpm for 24 hours, and then expanded in a fermenter with an inoculum size of 5% (v / v). Mix the bacterial agent and nutrient agent cultivated in the fermenter at a ratio of 1:1, pack into barrels, seal, and refrigerate at 2-8°C.

[0057] The culture medium for the expanded culture of the fermenter was prepared according to the following proportions: 5000L water, 50kg peptone, 25kg yeast powder, 50kg sodium chloride, 20kg soybean oil, pH 7.2, 121°C damp heat sterilization for 20min.

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Abstract

The present invention provides a new strain of oil-degrading bacteria-Aeromonas genus, Aeromonas allosaccharophila CY01 (Aeromonas allosaccharophila CY01) and the application of the strain. The bacterial strain of the present invention can rapidly multiply in the aerobic pool of the sewage treatment equipment, become a dominant bacterium, continuously secrete lipase, and degrade animal and vegetable oils.

Description

technical field [0001] The invention relates to a new bacterial strain—Aeromonas abnormal saccharophila CY01 (Allosaccharophila CY01), and a method for using the bacterial strain to degrade oil in sewage. Background technique [0002] With the development of my country's economy and the improvement of residents' living standards, the content of animal and vegetable oils in the daily diet is getting higher and higher, and a large amount of oily catering wastewater is directly discharged into the urban sewage pipe network and nearby water bodies without treatment, increasing the number of urban sewage treatment plants. The pollution load has caused severe eutrophication of the environmental water body. If the catering wastewater of some farmhouses and restaurants in tourist areas is not discharged up to the standard, it will seriously damage the ecological environment of the scenic spot. At present, the catering wastewater treatment technologies adopted at home and abroad can b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/02C02F3/34C12R1/01C02F101/34
CPCC02F3/02C02F3/34C02F2101/34C12N1/02C12N1/20C12N1/205C12R2001/01Y02W10/10
Inventor 郑展望阳波金鹏谢柳
Owner ZHEJIANG SHUANGLIANG SUNDA ENVIRONMENTAL PROTECTION CO LTD
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