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Cytoskeleton, islet cell compound carried by cell cytoskeleton, preparation method and application

A cell scaffold and islet cell technology, applied in the field of biomedicine, can solve the problems of hypoglycemia, inability to carry out effective treatment, limitation of treatment methods, etc., and achieve the effects of lowering blood sugar, enriching the source of islet cells, and improving the treatment effect.

Pending Publication Date: 2021-07-30
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the above methods can effectively treat patients with type 2 diabetes poisoning who have developed extensive drug resistance, and insulin therapy is still required for patients, but insulin therapy may bring the risk of hypoglycemia
[0006] Currently, treatment options for both type 1 and type 2 diabetes are still limited by lifelong medication and hypoglycemia

Method used

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  • Cytoskeleton, islet cell compound carried by cell cytoskeleton, preparation method and application
  • Cytoskeleton, islet cell compound carried by cell cytoskeleton, preparation method and application
  • Cytoskeleton, islet cell compound carried by cell cytoskeleton, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0062] Experimental Example 1 Mechanical properties, water content, swelling property, internal and external appearance and structure test of sodium alginate hydrogel

[0063] 1.1. Mechanics test method of sodium alginate hydrogel: Cut the prepared sodium alginate hydrogel into a cube with a width of 5 cm, a length of 5 cm, and a height of 5 cm, and measure the thickness with a vernier caliper. Use an electronic universal testing machine (INSTRON 5948) to check the elastic modulus of the sample taken. When measuring, fix the sample on the metal receiver. After fixing, the pressure sensor at the upper end moves axially towards the metal receiver at a constant speed of 1mm / min. Stress-strain curves of the two materials are obtained by extrusion until the material is deformed, and the elastic modulus is calculated from the slope of the linear part of the stress-strain curve. Three sets of parallel experiments were done for each material to explore the effect of sodium alginate co...

experiment example 2

[0076] Experimental Example 2 Effect of Sodium Alginate Hydrogel on the Proliferative Activity of Cell Lines

[0077] Experimental method: In order to evaluate the biological safety of sodium alginate hydrogel, the cck8 method was used to detect the effect of sodium alginate hydrogel on HUVEC cells, C2C12 cells, The effect of RINm5f cells and ADSCs cell proliferation activity.

[0078] The CCK8 method is a common method used to detect cell proliferation. The principle is that the reagent contains WST-8 [chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitro phenyl)-5-(2,4-disulfonic acid phenyl)-2H-tetrazole monosodium salt]. It is reduced to a highly water-soluble yellow formazan product (Formazan dye). The amount of formazan produced is directly proportional to the number of living cells. This feature can therefore be used directly for cell proliferation and toxicity assays. This experiment explored the effect of sodium alginate hydrogel on cell proliferation, so the cel...

experiment example 3

[0080] Experimental Example 3 Scaffold-mounted Cell Survival Detection of Transplanted Cells in Vivo

[0081] experimental method:

[0082] Build GFP + ADSCs cells: spread ADSCs in good growth state at passage 3 into 6-well plates, and ensure that the cell confluence rate reaches 70%-80% when virus infection is performed on the second day. GFP + The virus infection solution was added to the cells at a multiplicity of infection (MOI) = 100. After culturing for 6 hours, the virus-containing culture solution was aspirated, replaced with fresh complete culture solution, and the culture was continued at 37° C. for 48 hours, and the infected cells were harvested.

[0083] Skeletal muscle transplantation: Beijing Huafukang Biotechnology Co., Ltd. purchased 24 6-week-old male Wistar rats weighing 160-180g (certificate number [SCXK (Beijing) 2019-0008]), free to eat and drink, water, and temperature 25 ℃, humidity 55% to 65%, maintain 12h of light per day, day and night cycle, adapt...

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Abstract

The invention is applicable to the technical field of biomedicine, and provides a cytoskeleton, an islet cell compound carried by cell cytoskeleton, a preparation method and application. According to the preparation method of the cytoskeleton, the PDA-PLGA cytoskeleton is filled with the gel material, and the PDA-PLGA-Hydrogel cytoskeleton is obtained. The PDA-PLGA biological scaffold is adopted to wrap the hydrogel material to prepare the PDA-PLGA-Hydrogel cytoskeleton, and the PDA-PLGA-Hydrogel cytoskeleton not only has biocompatibility of a natural high polymer material, but also has strong mechanical property of a synthetic high polymer material, and has the potential of serving as a cell transplantation carrier; a PDX-1 transfection combined induction method is applied, so that adipose-derived stem cells are directionally induced into islet cells, insulin is continuously secreted, and the islet cells have the characteristic of glucose-dependent insulin secretion, so that abundant islet cell sources are provided for diabetes cell therapy; the PDA-PLGA-Hydrogel stent is used for carrying islet cells to transplant at skeletal muscle parts, has the characteristics of continuous insulin secretion and biodegradability, can effectively reduce blood sugar, and is proved to have a relatively good treatment effect on diabetes mellitus.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a cell scaffold, a complex of pancreatic islet cells loaded on it, a preparation method and an application. Background technique [0002] Diabetes mellitus (DM) is an inherited disease caused by the accumulation of glucose in the blood. Diabetes leads to pancreatic islet dysfunction, causing a series of comprehensive metabolic disorders related to sugar, protein, fat or electrolytes, while the appearance of hyperglycemia causes diabetes. Diabetes triggers a cascade of vascular events affecting most tissues and is a major cause of renal failure, vision loss, ischemic heart disease, stroke and peripheral arterial occlusive disease. [0003] DM is divided into type 1 DM and type 2 DM, in which type 1 DM destroys insulin-producing β cells (islet β cells) due to autoimmune reactions, while type 2 DM causes long-term changes in the peripheral function of insulin, there...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/39A61K47/34A61K47/36A61P3/10C12N5/071
CPCA61K35/39A61K47/34A61K47/36A61K9/0024A61P3/10C12N5/0676C12N2506/1384
Inventor 张明陈立邵丹张文友李晗嘉杨雪晗
Owner JILIN UNIV
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