Coprophilous fungus composition and preparation method and purpose thereof
A technology of composition and fecal bacteria, which is applied in the field of piglet breeding, can solve the problems of unfavorable large-scale promotion and poor effect of diarrhea prevention and control, and achieve the effects of enhancing feeding compliance, good rheological properties, and improving taste
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Embodiment 1
[0046] Screening method for donor pigs and feces
[0047] Adult pigs over 300 days old (regardless of sex), the candidate individuals have no clinical symptoms of diarrhea and other major pig diseases. No antibiotics were used within 30 days before stool collection, and clear immunization / medication records were available. Screening method of donor pigs: using serology, antibody detection and other means, referring to GB / T22914-2008 "Control and Monitoring of SPF Pig Pathogens" to exclude common / severe infectious pathogens in pigs.
[0048] Table 1. Screening method for donor pigs
[0049]
[0050] Screening of donor feces: Refer to GB29921-2013 "Pathogen Limits in Food" standard for Salmonella, Listeria monocytogenes, Escherichia coli O157:H7, Staphylococcus aureus, Vibrio parahaemolyticus 5 detection of pathogenic bacteria.
[0051] Abnormal toxicity test: According to the general rule 1141 of the Pharmacopoeia of the People's Republic of China, abnormal toxicity test ...
Embodiment 2
[0054] The preparation steps of fecal bacteria freeze-dried powder are:
[0055] Obtaining the fecal suspension:
[0056] Add 1 part of fresh feces of healthy adult pigs to 9 parts of sterile saline at 4°C, shake and mix with a homogenizer; Centrifuge for 5 minutes, carefully collect the supernatant; centrifuge the collected supernatant at 4600 rpm for 15 minutes at 4°C, and pour off the supernatant; the precipitate collected by centrifugation is in a 1:1 ratio, and again Add sterile physiological saline at 4°C, centrifuge at 4,600 rpm for 15 minutes at 4°C, and pour off the supernatant to obtain the final stool suspension.
[0057] Fecal bacteria concentration = (wet fecal weight / final fecal suspension volume) × 100% (unit: g / mL)
[0058] Addition of protective agent:
[0059] Add 10% sterilized glycerol (V / V) by volume to the final stool suspension, mix well and store at -80°C, add 10% sterilized skim milk equal to the volume of the final stool suspension , and then add ...
Embodiment 3
[0063] The stability test of the feces bacterium freeze-dried powder preparation of embodiment 2
[0064] After the freeze-dried powder of fecal bacteria was sealed at room temperature and stored in the dark for 0 month and 3 months, samples were taken to detect the contents of Bacillus, Lactobacillus, Lactobacillus, Bifidobacterium and Streptococcus thermophilus in the powder. Bacillus was detected by LB plate dilution coating method. Lactobacillus, Lactobacillus, Bifidobacterium and Streptococcus thermophilus were detected by the national standard GB4789.35-2010 method. 5 batches were randomly selected, and the average value of the test results was calculated. The results are shown in Table 2:
[0065] Table 2
[0066]
[0067] Conclusion: After the fecal bacteria freeze-dried powder prepared in Example 2 was stored at room temperature and protected from light for 3 months, the number of viable bacteria remained at 10 7 More than CFU / g, it shows that the present invent...
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