Extraction and composition analysis method for organic phosphorus in lake aquatic plants

Abstract

The invention discloses an extraction method for organic phosphorus in lake aquatic plants. The extraction method comprises the steps of sample collection, phosphoric basic composition determination, organic phosphorus (Po) extraction, sample testing pretreatment and liquid nuclear magnetism (31P-NMR) analysis and calculation. According to the method, the optimal Po extraction effect is obtained by adopting NaOH with the concentration of 0.5 mol / L and an EDTA extraction agent with the concentration of 25 mmol / L on the condition that the solid-to-liquid ratio of aquatic plant powder to the extraction agent is 1:60; in the 31P-NMR analysis and test process, the delay time (D1) is set as 5 s, the scanning and analyzing time is set as 15 h, and a good spectrogram can be obtained. The aquatic plant Po extraction method has the advantages that the average extraction rate of total phosphorus (TP) and Po is 91.4% or above and 88.1% or above respectively, the specific variety and content of Po in a sample can be accurately detected, and the composition structure characteristics of Po in the lake aquatic plants can be accurately reflected; a scientific basis is provided for research on the biogeochemical characteristics of Po in the lake aquatic plants and contribution of Po in algal bloom.

Description

technical field [0001] The invention relates to a method for extracting and analyzing the composition of organic phosphorus, in particular to a method for extracting and analyzing the composition of organic phosphorus in aquatic plants in lakes. Background technique [0002] Phosphorus is one of the key limiting nutrients in aquatic ecosystems and an important factor affecting lake eutrophication. Exogenous input and endogenous circulation are the main factors leading to the substantial increase of phosphorus in lake water. In recent years, the input of exogenous phosphorus, including domestic sewage, industrial and agricultural production, and soil runoff, has been controlled to a certain extent, and the release of endogenous phosphorus in lakes has become a key factor causing further deterioration of lake water quality. Sediment release is one of the important sources of endogenous phosphorus pollution in lakes, but the products of death and decomposition of aquatic plant...

AI Technical Summary

Problems solved by technology

What this invention discloses is the extraction method of deposit, and its shortcoming is that the extraction rate is not high

CN104614331A discloses a method for simultaneously extracting and analyzing the forms of organic phosphorus and inorganic phosphorus in water sediments to solve the problem that the existing phosphorus form extraction method cannot simultaneously measure the content of organic phosphorus and inorganic phosphorus in water sediments

However, there are few reports on the extraction and composition analysis of organic phosphorus in aquatic plants in lakes. Therefore, the research on the extraction and composition analysis of organic phosphorus in aquatic plants in lakes is very important for the exploration of P in aquatic plants in lakes. o The contribution made in the process of algal blooms and the lake's endogenous phosphorus cycle and mechanism are of great scientific significance

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