Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

An extraction and composition analysis method for lake alga organophosphorus

An extraction method, an organic phosphorus technology, is applied in the direction of analysis by nuclear magnetic resonance, preparation of test samples, etc., and can solve the problem of low extraction efficiency

Inactive Publication Date: 2016-10-26
CHINESE RES ACAD OF ENVIRONMENTAL SCI
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned literature mainly discloses that P in the sediment or granular state in the water body o extraction process, and P o The extraction efficiency is not high, which has a certain impact on the accurate understanding of the biogeochemical cycle of aquatic ecosystems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An extraction and composition analysis method for lake alga organophosphorus
  • An extraction and composition analysis method for lake alga organophosphorus
  • An extraction and composition analysis method for lake alga organophosphorus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] A method for extracting organic phosphorus from lake algae, comprising the following steps:

[0032] (1) Sample collection: collect floating algae on the surface of eutrophic lake water, put them in a sealed bag for refrigerated storage, transport them back to the laboratory for freeze-drying, grind them thoroughly after freeze-drying, and pass through a 2mm sieve to obtain algae powder samples, which are frozen at -20°C Save for later use;

[0033] (2) Determination of the basic composition of phosphorus: total phosphorus TP, inorganic phosphorus P i and P o the determination of

[0034] Accurately weigh two samples of 0.5g algae powder, each with 3 parallel samples, one was calcined in a muffle furnace at 450°C for 3 hours, and transferred to a centrifuge tube after cooling down to room temperature, and 20 mL of 3.5 mol / L HCl was added to shake for 16 hours. After centrifugation, the supernatant was filtered with a 0.45 μm filter membrane to obtain the extract, dig...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

An extraction and composition analysis method for lake alga organophosphorus is disclosed. The method includes steps of sample collection, measurement of phosphorous basic composition, organophosphorus (Po) extraction, sample testing pretreatment, liquid phosphorus nuclear magnetic resonance (<31>P-NMR) analysis and calculation. A mixture of 0.5 mol / L of NaOH and 25 mmol / L of EDTA is adopted as an extractant, and the solid liquid ratio of an alga powder sample to the extractant is 1:60 in the method to obtain an optimum Po extraction effect. During <31>P-NMR analysis testing, delay time (D1) is set to be 5 s and scanning analysis time is 15 h so that a good spectrum can be obtained. The average extraction rates of total phosphorous (TP) and the Po in the method are respectively 95.0% and 94.9% or above. The method can accurately detect specific varieties and contents of the Po in the sample, and can truly and accurately reflect composition characters of the Po in the lake algae. The method provides important scientific bases for researching contributions of lake algae in an algal bloom process, for researching lake endogenous phosphorus circulation processes and for government decision-making.

Description

technical field [0001] The invention relates to a method for extracting and analyzing the composition of organic phosphorus, in particular to a method for extracting and analyzing the composition of organic phosphorus in lake algae. Background technique [0002] Phosphorus is one of the key elements in the metabolism of freshwater lake ecosystems. Excessive phosphorus will lead to the growth and reproduction of algae, causing deterioration of water quality and endangering the safety of ecosystems. Exogenous input and endogenous circulation are the main factors leading to the substantial increase of phosphorus in lake water. In recent years, the input of exogenous phosphorus, including domestic sewage, industrial and agricultural production, and soil runoff, has been controlled to a certain extent, and the release of endogenous phosphorus in lakes has become a key factor causing further deterioration of lake water quality. Sediment release is one of the important sources of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N24/08G01N1/34
CPCG01N24/08G01N1/34
Inventor 冯伟莹吴丰昌朱元荣张琛
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com