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Method for quenching fluorescence by using nanometer silver sol in Raman spectroscopy

A nano-silver sol and Raman spectroscopy technology, applied in the field of analysis and detection, can solve problems such as infeasibility, and achieve the effect of simple and easy sample preparation method

Inactive Publication Date: 2018-09-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Sometimes, however, adding a quencher is not feasible (such as for bulk samples), so other methods must be used to overcome fluorescence interference

Method used

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  • Method for quenching fluorescence by using nanometer silver sol in Raman spectroscopy
  • Method for quenching fluorescence by using nanometer silver sol in Raman spectroscopy
  • Method for quenching fluorescence by using nanometer silver sol in Raman spectroscopy

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The samples in this embodiment are epoxy resin boards. The sample is a commercial product with a yellow appearance and a thickness of 1 mm. Through infrared spectrum analysis, it is confirmed that the polymer therein is indeed an epoxy resin, and the main component of the filler is calcium carbonate (CaCO 3 ), and a small amount of glass fiber. Pure epoxy resin shows strong fluorescence in Raman spectrum, and the sample shows strong fluorescence due to the high content of filler in this product. Carry out Raman spectrum detection to the sample of this embodiment, implementation method is as follows:

[0023] (1) Sample preparation:

[0024] Saw a small piece of 0.5mm×0.5mm from the plate with a saw blade as the sample to be tested. Wipe clean surface with roll paper.

[0025] (2) Add nano-silver sol dropwise:

[0026] For bulk samples, drop a drop of nano-silver sol on the sample to keep the liquid in a hemispherical shape. Let it stand for half a minute and wait...

Embodiment 2

[0031] The sample of this embodiment is graphene, and its appearance is black powder. Due to the use of certain additives in the preparation process, the sample showed strong fluorescence, and the D peak, G peak and 2D peak of graphene could not be observed. Carry out Raman spectrum detection to the sample of this embodiment, implementation method is as follows:

[0032] (1) Sample preparation:

[0033] Take a small amount of sample and place it on a glass slide, add 2 to 3 drops of nano-silver sol dropwise on it, stir gently with a disposable pipette to mix evenly, and let it stand for 1 min to be tested.

[0034] (2) Raman spectrum detection:

[0035] The sample is placed in the reflected light path of the Raman spectrometer for detection. The instrument conditions are: excitation wavelength 532nm, power to the sample 0.6mW, 10X lens, grating 600gr / mm, pinhole 400μm, slit 100μm, accumulation time 10s, accumulation 4 times. When selecting a point, pay attention to selecti...

Embodiment 3

[0038] The present embodiment sample is carbon nitride (C 3 N 4 ), the appearance is a yellow powder, which is prepared by heating dicyandiamide at high temperature in a muffle furnace. Due to the control of the process, the carbon nitride sample only shows strong fluorescence, which is different from the usual carbon nitride samples that show extremely strong fluorescence. Carry out Raman spectrum detection to the sample of this embodiment, implementation method is as follows:

[0039] (1) Sample preparation:

[0040] Take a small amount of sample and place it on a glass slide, add 2 to 3 drops of nano-silver sol dropwise on it, stir gently with a disposable straw to mix evenly, and let it stand for 2 minutes to be tested.

[0041] (2) Raman spectrum detection:

[0042] The sample is placed in the reflected light path of the Raman spectrometer for detection. The instrument conditions are: excitation wavelength 785nm, power to the sample 30mW, 50X telephoto lens, grating ...

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Abstract

The invention belongs to the technical field of analytical detection, and discloses a method for quenching fluorescence by using a nanometer silver sol in Raman spectroscopy. The method comprises: adding a nanometer silver sol to the surface of a to-be-detected sample in a dropwise manner, or mixing a nanometer silver sol and a to-be-detected sample; placing the sample in the reflection light pathof a Raman spectrometer; and carrying out Raman spectrum detection. With the method of the present invention, the interference caused by fluorescence can be eliminated to a certain extent, the good spectrum of the substance with strong fluorescence can be obtained, and the method is especially suitable for powdery substances, is simple and effective and suitable for rapid detection; and the peakof the spectrum is not enhanced during the use of the method, such that the results of the method can be generally compared to the results of the normal Raman experiments.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a method for quenching fluorescence with nano-silver sol in Raman spectroscopy. Background technique [0002] Indian physicist C.V.Raman and others discovered a new scattering phenomenon of visible light in 1928. When this kind of scattering occurs, the frequency of scattered light will change to a certain extent. This phenomenon is called Raman scattering. . Raman scattering is produced by phonons such as optical phonons of matter. Raman was awarded the Nobel Prize in Physics in 1930 for his discovery of the Raman effect and Raman scattering. The Raman phenomenon is an inelastic scattering phenomenon of light. In this process, photons and phonons exchange energy, which can be explained by Raman's "virtual energy level". In a conventional Raman experiment, the incident light energy is relatively low, and the scattering that occurs is called normal Ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 梁庆优黄思静宋国胜杨贤锋崔洁
Owner SOUTH CHINA UNIV OF TECH
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