A method for enzymatically preparing 1,3-dioleic acid-2-palmitic acid triglyceride in a subcritical system
A technology for palmitic acid triglyceride and enzymatic preparation, which is applied to the preparation of 1,3-dioleic acid-2-palmitic acid triglyceride, the field of food additives and breast milk fat substitutes, and can solve the problem of lack of efficient enzymatic reaction system, troubled OPO large-scale production, high OPO cost, etc., to achieve the effects of improving reaction rate and conversion rate, reducing equipment investment and operating costs, and low acyl transfer rate
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[0025] In the present invention, the lipase in the immobilized form, referred to as immobilized lipase, can be commercially available LipozymeRM IM, Lipozyme TL IM, etc., and can also be prepared by itself, and its preparation method includes the following steps:
[0026] 1) Activation of the carrier: Soak in 1mol / L hydrochloric acid solution to remove impurities such as metal ions in the carrier, then wash with pure water until neutral; 2) Adsorption immobilization: prepare a buffer solution, dissolve free lipase in the buffer solution, and then Add silica gel, resin, diatomaceous earth and other carriers, vacuum and stir for 0.5-4 hours, and collect the crude immobilized lipase by suction filtration; 3) Washing and drying: wash the crude immobilized lipase with buffer solution for 3 times, freeze-dry or The immobilized lipase product can be obtained by cold air drying.
Embodiment 1
[0029] A method for enzymatically preparing 1,3-dioleic acid-2-palmitic triglyceride in a subcritical state, comprising the steps of:
[0030] (1) Preparation of raw material A: 52% palm oil stearin was used as raw material, sodium methoxide was used as a catalyst, and the amount of sodium methoxide added was 1% of the mass of 52% palm oil stearin, reacted at a reaction temperature of 80°C for 1 h, and then used Aqueous citric acid solution to remove sodium methoxide, and rotary evaporation to remove water, can obtain recombinant palm oil with a mass content of palmitic acid at the sn-2 position of not less than 60%, that is, raw material A;
[0031] Raw material B selects oleic acid, which is derived from the hydrolysis of high oleic rapeseed oil;
[0032](2) Subcritical enzyme-catalyzed transesterification reaction: Add 500g of raw material A, 500g of raw material B, and 30g of diatomaceous earth-immobilized Candida lipolytica lipase into the autoclave, add 5L of liquefied p...
Embodiment 2
[0035] A method for enzymatically preparing 1,3-dioleic acid-2-palmitic acid triglyceride in a subcritical state, comprising the steps of:
[0036] (1) Preparation of raw material A: 52% palm oil stearin was used as raw material, sodium ethoxide was used as a catalyst, and the amount of sodium ethoxide added was 2% of the mass of 52% palm oil stearin, reacted at a reaction temperature of 100°C for 0.5h, and then used Aqueous citric acid solution to remove sodium ethoxide, and rotary evaporation to remove water, can obtain the recombinant palm oil with the mass content of palmitic acid at the sn-2 position not less than 60%, that is, raw material A;
[0037] Raw material B chooses oleic acid, which comes from the hydrolysis of olive oil;
[0038] (2) Subcritical enzyme-catalyzed transesterification reaction: Add 500g of raw material A, 1000g of raw material B, 75g of silica gel-immobilized Candida antarctica lipase (5% of the total mass of raw materials) into the autoclave, and...
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