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Double-sided amphiphilic carrier, as well as preparation method and application thereof

An amphiphilic and carrier technology, which is applied to the double-sided amphiphilic carrier and its preparation and application fields, can solve the problems of neglecting the influence and the like, and achieve the effects of improving catalytic activity, improving environmental adaptability and expanding production.

Active Publication Date: 2020-01-07
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of enzymes, people immobilized enzymes in different two-dimensional carrier materials to improve the catalytic activity of enzymes, such as graphene, carbon nitride (C 3 N 4 ), fossil hydrate (LDH), molybdenum sulfide (MoS 2 ), etc. These two-dimensional materials have high specific surface area and hydrophobicity, which is beneficial to the mass transfer process in the catalytic process. In addition, the hydrophobic structure is conducive to the expression of enzyme activity ("open" form), but these reports only pay attention to the orientation of the enzyme. In terms of the influence on the enzyme activity, the influence of the reaction medium on the enzyme is ignored

Method used

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  • Double-sided amphiphilic carrier, as well as preparation method and application thereof
  • Double-sided amphiphilic carrier, as well as preparation method and application thereof
  • Double-sided amphiphilic carrier, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] C / MoS 2 Preparation of carrier material:

[0034] After fully mixing 4g of dicyandiamide and 0.1g of glucose, place it in a tube furnace for calcination. The calcination procedure is to raise the temperature from room temperature to 600°C and then to 800°C in a nitrogen atmosphere, keep it warm for 1 hour, and drop to room temperature to obtain C Material.

[0035] Disperse 0.165g of material C in 165ml of UP water and ultrasonically disperse for 0.5h, then fully dissolve 0.75g of sodium molybdate in the above aqueous dispersion of C, add 3.125g of L-cysteine ​​and ultrasonically disperse for 1h, and place the above mixed solution Put it in a reaction kettle, and react at 220°C for 24h. After the reaction, it will be cooled to room temperature, washed with ethanol for several times, and then dried in a vacuum oven at 60°C to obtain C / MoS 2 . The resulting C / MoS 2 C:MoS in materials 2 The mass ratio is 1:3.

[0036] Preparation of C / MoS 2Immobilized enzyme: 1) Dis...

Embodiment 2

[0043] C / MoS 2 Preparation of carrier material:

[0044] Put 4g of dicyandiamide in a tube furnace for calcination. The calcination procedure is to raise the temperature from room temperature to 550°C in a nitrogen atmosphere and keep it for 4h, then cool down to room temperature naturally. The obtained carbon nitride raw material is placed in an aqueous solution of glucose , sonicated for 4 h and then freeze-dried. The freeze-dried mixture was placed in a tube furnace, heated from room temperature to 800 ° C under a nitrogen atmosphere and kept for 1 h, and then cooled to room temperature to obtain carbon materials.

[0045] Then fully dissolve 0.165g of sodium molybdate in the water dispersion of C, add 0.11g of thiourea and sonicate for 1h, put the above mixed solution in the reaction kettle, and react at 240°C for 18h. After washing with ethanol several times, dry in a vacuum oven at 60°C to obtain C / MoS 2 . The resulting C / MoS 2 C:MoS in materials 2 The mass ratio is...

Embodiment 3

[0048] C / MoS 2 Preparation of carrier material:

[0049] After fully mixing 4g of melamine and 0.5g of glucose, place it in a tube furnace for calcination. The calcination procedure is to raise the temperature from room temperature to 650°C and then to 850°C in a nitrogen atmosphere, keep it warm for 1 hour, and then lower it to room temperature to obtain material C.

[0050] Dissolve 0.6g of ammonium thiosulfate in 20ml of UP water and disperse ultrasonically for 0.5h, then disperse the prepared C material solution in the above solution, disperse ultrasonically for 1h, put the above mixed solution in a reaction kettle, and react at 240°C After 18 hours, after the reaction was completed, it was cooled to room temperature, washed with ethanol for several times, and then dried in a vacuum oven at 60°C to obtain C / MoS 2 . The resulting C / MoS 2 C:MoS in materials 2 The mass ratio is 1:3.

[0051] Preparation of C / MoS 2 Immobilized enzyme: 1) 3g Rhizomucor miehei lipase (RML)...

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Abstract

The invention relates to a two-dimensional carbon nano carrier material as well as a preparation method and application thereof. The double-sided amphiphilic carrier provided by the invention comprises hydrophilic layer-shaped multilayer carbon nanosheets and a metal sulfide, and the metal sulfide is selectively modified on one side of each single-layer nanosheet. The invention aims to solve the technical problems in the prior art. The double-sided amphiphilic carrier provided by the invention has a hydrophilic interface and a hydrophobic interface at the same time; with maintained enzyme loading capacity, the carrier can stably exist on an interface of an oil phase and a water phase, interface catalysis of enzymes is facilitated, the enzyme catalytic activity is improved, meanwhile. The invention further provides a preparation method and application of the carrier, and the carrier can be used as a carrier of immobilized enzymes.

Description

technical field [0001] The invention relates to a two-dimensional carbon nanocarrier material and its preparation method and application. Background technique [0002] As a biocatalyst with special catalytic functions, enzymes are widely used in bioengineering, food industry, medicine, fine chemical industry and other fields due to their high catalytic efficiency, high specificity and mild reaction conditions. However, there are still many problems in the application of free enzymes, for example, the enzymes are easily inactivated or denatured during storage and reaction, resulting in poor operational stability and reusability. In addition, the enzyme is sensitive to the state of the reaction medium, such as the nature of the solvent, reaction temperature, pH, etc. Changing the conditions will greatly affect the activity of the free enzyme. Therefore, in order to improve the stability and operability of free enzymes, people have conducted in-depth studies on the properties ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/15C01G39/06C12N11/14
CPCC01G39/06C01P2002/88C01P2004/04C12N11/14C01B32/15
Inventor 黄凤洪时杰张珊邓乾春万楚筠周琦刘昌盛郑畅
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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