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Application of steroid saponin RCE-4 in preparation of medicines for preventing or treating tumors

A technology of RCE-4 and steroidal saponins, applied in the field of medicine, can solve problems such as toxic side effects and high costs

Inactive Publication Date: 2015-11-04
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surgery, radiotherapy and chemotherapy are still the main means of clinical tumor treatment, among which chemotherapy is one of the important means, but its high cost and serious side effects have become the bottleneck limiting its clinical application

Method used

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  • Application of steroid saponin RCE-4 in preparation of medicines for preventing or treating tumors
  • Application of steroid saponin RCE-4 in preparation of medicines for preventing or treating tumors
  • Application of steroid saponin RCE-4 in preparation of medicines for preventing or treating tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] RCE-4, a compound with anti-tumor activity in Auspicious Grass, is specifically prepared by the following method:

[0055] 1) Pretreatment of auspicious grass

[0056] Auspicious grass rhizomes were washed and dried in the sun, and 0.005 kg was taken for preservation, and the rest was dried in a constant temperature blast box at 48°C until constant weight, net weight 4.0 kg.

[0057] 2) Auspicious grass reflux extraction

[0058] Cut the sample into small pieces, use 95% ethanol as solvent, and extract under normal pressure at 65°C. After the extract is filtered, it is concentrated with a rotary evaporator at 55°C, and then evaporated to dryness in a fume hood until there is no alcohol smell. , to obtain a deep extract, weighed to obtain 1.4kg of extract (see figure 1 ).

[0059] 3) Extraction of Auspicious Grass Extract

[0060] Add 1 liter of distilled water to dissolve the extract obtained in 2) above, and extract with extractant sherwood oil, ethyl acetate, and ...

experiment example 1

[0083] The application of the RCE-4 obtained in Example 1 of the present invention for anti-liver cancer at the cellular level will be described below in conjunction with specific experimental examples.

[0084] Effect of RCE-4 on normal cells monkey kidney cells (marc-145), canine kidney cells (MDCK) and human liver cancer cell H 22 and the cytotoxic activity of HepG2.

[0085] experiment method

[0086] 1) Cell culture

[0087] marc-145 and MDCK cells were cultured in DMEM-F12 medium in CO 2 5% CO in the incubator 2 , 37°C and saturated humidity. When the cell monolayer grows to 80%, it is digested with 0.25% trypsin containing EDTA and passaged.

[0088] h 22 and HepG2 human liver cancer cells were cultured in RPMI-1640 medium in CO 2 Cultivate in an incubator with 5% CO2, 37°C and saturated humidity. When the cell monolayer grows to 80%, it is digested with 0.25% trypsin and subcultured.

[0089] 2) 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) ...

experiment example 2

[0099] The application of RCE-4 in Example 1 of the present invention in anti-hepatoma at the animal level will be described below in conjunction with specific examples.

[0100] RCE-4 for H 22 Antitumor activity in tumor-bearing mice.

[0101] RCE-4 for H 22 Anti-tumor test in mice with ascites tumor

[0102] experiment method

[0103] 1) Experimental materials

[0104] Female Kunming mice (18-22 g) were purchased from the Experimental Animal Center of China Three Gorges University, animal production license number: SCXK (E) 2011-0012. The H22 tumor cell line was purchased from the Chinese Type Culture Collection Center of Wuhan University, and was preserved by the Hubei Provincial Key Laboratory of Natural Product Research and Utilization of Three Gorges University.

[0105] 2) Establishment of cell culture and animal model

[0106] h 22 Cells were cultured in 10% newborn calf serum, 10 5 U / L penicillin, 100 mg / L streptomycin in RPMI-1640 medium, placed at 37 ° C, CO...

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PUM

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Abstract

The invention discloses an application of steroid saponin RCE-4 separated from pink reineckea herb in the preparation of medicines for preventing or treating tumors. The separated RCE-4 has weaker cytotoxicity to normal cells but has stronger cytotoxicity to human hepatoma carcinoma cells H22 and HepG2, has remarkable anti-tumor effects on both mice with H22-bearing ascitic tumor and solid-tumor, and has no obvious side-effect on routine blood indexes, blood biochemical indexes and histomorphology. In comparison with existing anti-cancer drugs used in clinic, steroid saponin RCE-4 has equivalent effects but small toxic and side effect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the use of a compound steroidal saponin RCE-4 with antitumor activity in the preparation of medicines for preventing or treating tumors. Background technique [0002] Auspicious grass (Reineckia carnea (Andr.) Kunth.) is a single-species perennial evergreen herb of the genus Reineckia carnea of ​​Lily of the Lily family. Its nature and flavor are sweet and flat, and it has the effects of moistening lung and relieving cough, regulating blood, detoxification, nourishing kidney and setting bones, dispelling wind and dehumidification, and is mainly used for tuberculosis, cough and hemoptysis, chronic bronchitis, asthma, rheumatoid arthritis, external use for bruises, The treatment of fractures and other diseases is regarded as "sacred grass" by Tujia and Miao folks. According to analysis, its main chemical components include: steroidal saponins, flavonoids, triterpenoids...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048C07J71/00A61P35/00
Inventor 贺海波邹坤杨小姣刘呈雄白彩虹王倩汪鋆植郭志勇薛艳红杨进
Owner CHINA THREE GORGES UNIV
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