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Method for preparing bacterial cell catalyst for catalyzing aesculin transesterification

A bacterial cell and exchange reaction technology, which is applied in the field of bacterial cell catalyst preparation, can solve the problems of low substrate utilization and low product purity, and achieve the effects of simple and easy-to-operate preparation method, high regioselectivity, and easy cultivation

Inactive Publication Date: 2015-06-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims at the shortcomings of existing catalysts for the synthesis of fennecine esters, and aims to provide a novel method for preparing a bacterial catalyst that catalyzes the synthetic reaction of fendecine esters
The prepared bacterial cell catalyst can selectively catalyze the synthesis of 6′-monoester of fenpetrin, and the regioselectivity is higher than 90%, thereby overcoming the low selectivity of traditional chemical methods, which leads to low substrate utilization, low product purity, and easy Disadvantages such as generation of by-products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] will include Pseudomonas aeruginosa Pseudomonas aeruginosa GIM1.46 The seed liquid was transferred to a liquid medium for culture, the inoculation amount was 0.5% (v / v), the culture condition was 25°C, and the composition of the liquid medium was: 10 g / L soybean oil, 1 g / L yeast extract, 0.2 g / L MgSO 4 ·7H 2 O , 5 g / L (NH 4 ) 2 SO 4 , 1 g / L K 2 HPO 4 , pH 7.0. After culturing for 60 hours, the wet cells were collected by centrifugation, washed twice with distilled water, and vacuum freeze-dried to obtain the cell catalyst. The cell catalyst was used in the transesterification reaction of fendecine and vinyl propionate, and the 6''-fendecine propyl ester was 95.3%.

[0021] The regioselectivity of the catalyst obtained by the method of the present invention is determined by the regioselective acylation reaction of fenceretin hemihydrate and vinyl propionate, and the reaction condition is to add 40 mg of thalline to the 2mL fracteretin esterification reaction sys...

Embodiment 2

[0023] will include Pseudomonas aeruginosa Pseudomonas aeruginosa GIM1.46 The seed liquid was transferred to a liquid medium for culture, the inoculation amount was 0.5% (v / v), the culture condition was 60°C, and the liquid medium composition was: 10 g / L Tween 80, 1 g / L yeast extract, 0.2 g / L MgSO 4 ·7H 2 O , 5 g / L (NH 4 ) 2 SO 4 , 1 g / L K 2 HPO 4 , pH 7.0. After culturing for 60 hours, the wet cells were collected by centrifugation, washed twice with distilled water, and vacuum freeze-dried to obtain the cell catalyst. The cell catalyst was used in the acylation reaction of fenceretin hemihydrate and vinyl propionate, and the regioselectivity of 6''- frenetin propyl ester was 95.4%.

Embodiment 3

[0025] will include Pseudomonas aeruginosa Pseudomonas aeruginosa GIM1.46 The seed solution was transferred to a liquid medium for culture, the inoculum size was 25% (v / v), the culture condition was 30°C, and the liquid medium composition was: 10 g / L Tween 80, 10 g / L yeast extract, 0.2 g / L MgSO 4 ·7H 2 O , 5 g / L (NH 4 ) 2 SO 4 , 1 g / L K 2 HPO 4 , pH 6.8. After culturing for 12 hours, the wet cells were collected by centrifugation, washed twice with distilled water, and vacuum freeze-dried to obtain the cell catalyst. The cell catalyst was used in the acylation reaction of aurecetin hemihydrate and vinyl propionate, and the regioselectivity of 6''-auretin propyl ester was 98.1%.

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Abstract

The invention discloses a method for preparing a bacterial cell catalyst for catalyzing the aesculin transesterification. The method comprises the following steps: inoculating a bacterium seed solution into a liquid culture medium according to the inoculation amount of 0.5-25% (v / v), and culturing for 12-96h at the temperature of 25-60 DEG C; and washing the bacterium body by the distilling water and performing vacuum freeze drying to harvest a bacterium dry powder, namely the bacterial cell catalyst. The catalyst is used for high selectively catalyzing the aesculin and an acyl reagent to generate the transesterification. Compared with an enzymic preparation, the bacterial whole cell catalyst obtained from the invention avoids the expensive and miscellaneous enzyme separation and purification processes, has the characteristics of easy preparation and recovery and is beneficial for the scale production; and the catalyst is stable in an organic solvent.

Description

technical field [0001] The invention belongs to the fields of microorganism culture, biocatalysis and biomedicine preparation, and relates to a preparation method of a bacterial cell catalyst for catalyzing the transesterification reaction of afraetin. Background technique [0002] Flavonoids are a series of important polyphenolic compounds formed by connecting two benzene rings through three carbon atoms. They are widely found in almost all plants, especially vegetables and fruits; they are widely used in medicine, food and cosmetics. Great application value. Flavonoids have a variety of biological activities including antioxidant activity, anti-inflammatory, anticancer, antibacterial, antiallergic, and antiviral, etc., however, most of the biological activities of flavonoids are limited by their low lipid solubility due to their polyhydroxy structure. or water soluble. The hydroxyl group located on the benzene ring or the glycoside skeleton has an important influence on ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/60C12R1/38C12R1/385
CPCC12N1/20C12P19/60
Inventor 李晓凤赖学能赵光磊吴晖余以刚
Owner SOUTH CHINA UNIV OF TECH
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