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Advanced methods for sugar production from lignocellulosic biomass and fermenting sugars to microbial lipids

A technology of lignocellulose and raw materials, applied in the field of microbial oils

Inactive Publication Date: 2015-04-01
WASHINGTON STATE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it requires heterogeneous cultivation that requires an organic carbon source

Method used

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  • Advanced methods for sugar production from lignocellulosic biomass and fermenting sugars to microbial lipids
  • Advanced methods for sugar production from lignocellulosic biomass and fermenting sugars to microbial lipids
  • Advanced methods for sugar production from lignocellulosic biomass and fermenting sugars to microbial lipids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] Analysis of Sugars in Wheat Grass Treated with Controlled Ozone and Ammonia Soaking (SAA) Treatments and a Combination of Both (Ozone Treatment followed by Ammonia Soaking Treatment)

[0119] To contrast and through 2 hours ozone treatment (state: (ozonation: 90% humidity is adjusted to 42-60 mesh particle diameter, 5.4% ozone reacts 2 hours under the air flow of 2 liters / min), only ammonia immersion handles (condition 60 ℃ and 24 hours), combined treatment (ozonation: 90% humidity is adjusted to 42-60 mesh particle size, 5.4% ozone reacts 10 minutes under the air flow of 2 liters / minute; Ammonia soaking: 20% solid content (w / v), 20% ammonium hydroxide aqueous solution (w / v), 50 ℃ of reaction temperature and the reaction time of 6 hours) the sugar analysis result of the rice straw sample is shown in table 1.The concentration of monosaccharide in the sample after ozone treatment The concentration decreases with time, which may be related to the reaction of ozone with su...

Embodiment 2

[0124] Effect of Ozone Pretreatment on Sugar Recovery of Wheat Straw Particle Size

[0125] Wheat straw particle size (42-60 mesh) was adjusted to a moisture content of 90% (W / W), and was heated at room temperature (23°C) with an ozone-enriched oxygen stream (oxygen flow rate 2 liters / min) containing 5.4% ozone. , React in a stainless steel reactor for 5 to 30 minutes. The treated biological raw material is taken out and saccharified according to the following procedure. The wheat straw residue (0.2 g) obtained from the above procedure was added to 20 ml of citrate buffer (pH=4.8) while adding 1.5 L (71.0 μL, protein concentration = 126.5 mg / mL) and Novozym 188 (14.0 μL, protein concentration = 134 mg / mL) enzyme mixture, and the mixture stayed on the shaker at 50°C for stirring. Samples were taken after 72 hours, enzymes were inactivated by boiling water for 5 minutes, and sugar concentrations were analyzed by Diane ion chromatography. The results are shown in Table 2. Th...

Embodiment 3

[0130] Effect of superoxide water treatment on enzymatic hydrolysis of wheat straw granules

[0131] 3 grams of wheat straw particles (42-60 mesh) are thoroughly mixed with water containing hydrogen peroxide (the molar ratio of hydrogen peroxide and ozone provided by concentration adjustment is 1:2) and adjusted to a moisture content of 90% (W / W). The mixture was placed in a stainless steel autoclave and generated highly reactive hydroxyl radicals at room temperature (23° C.) for 5 to 30 minutes with an ozone-enriched oxygen stream (oxygen flow rate 2 L / min) containing 5.4% ozone. The treated biological raw material is taken out and saccharified according to the following procedure. The wheat straw residue (0.2 g) obtained from the above procedure was added to 20 ml of citrate buffer (pH=4.8) while adding 1.5 L (71.0 μL, protein concentration = 126.5 mg / mL) and Novozym 188 (14.0 μL, protein concentration = 134 mg / mL) enzyme mixture, and the mixture stayed on the shaker at 50...

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Abstract

Methods for facilitating sugar release from lignocellulosic biomass and for utilizing the sugars for microbial lipid (e.g. biofuel) production are provided. The methods involve pretreating lignocellulosic biomass using various oxidizing agents (ozone, peroxone, etc.) at a temperature not higher than 50 DEC and pressure no higher than 1.5 atm to render the biomass more accessible to enzymatic hydrolytic degradation into sugars and utilizing soluble sugars for fermenting oleaginous microorganism to produce microbial lipids.

Description

technical field [0001] This invention is about a method to efficiently release sugars from lignocellulosic raw materials and use these sugars as raw materials for fermentation to produce bioenergy and biochemicals, such as microbial oils. The special feature of this invention is that it not only provides the pretreatment of lignocellulosic raw materials, but also hydrolyzes the pretreated biomass with different enzymes under high solid content, and uses the enzymatic hydrolysis liquid containing sugar released from the fiber raw materials to ferment and produce The production of microbial oil by oleaginous yeast is the step-by-step hydrolysis and fermentation method of lignocellulosic raw materials, and it also includes the method of producing microbial oil by synchronous saccharification and fermentation (that is, the enzymatic hydrolysis of pretreated fiber raw materials and the fermentation of oleaginous microorganisms are carried out simultaneously). Background technique ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P7/64
CPCC10L1/026C12P19/02C12P19/14C12P2201/00Y02E50/10Y02P20/582D21C1/08D21C11/10
Inventor 布鲁·马赫什高·艾伦陈树林
Owner WASHINGTON STATE UNIVERSITY
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