Chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment and expression and application thereof

Abstract

The invention relates to a chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment, as well as expression and application thereof, belonging to the fields of genetic engineering technologies, antibodies and kits. According to the chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment provided by the invention, a strong antigen epitope, namely 133 amino acids in total from 745th amino acid to 877th amino acid in the staphylococcus aureus surface protein FnBPA are screened out through computer analysis, a codon preferred by prokaryotes is selected, a brand new gene sequence of the antigen epitope is chemically synthesized, the gene fragment is expressed by utilizing the genetic engineering technology, and the strong antigen epitope fragment of the staphylococcus aureus surface protein FnBPA is prepared. The expressed strong antigen epitope fragment of the staphylococcus aureus surface protein FnBPA can be used for detection of staphylococcus aureus antibodies and immune preparation of anti-staphylococcus aureus monoclonal antibodies, polyclonal antibodies and the like.

Description

technical field [0001] The invention relates to the chemically synthesized surface protein FnBPA gene fragment of staphylococcus aureus and its expression and application, and uses genetic engineering technology to prepare the surface protein FnBPA of recombinant staphylococcus aureus. Through computer analysis, FnBPA fragments containing strong antigenic epitopes are screened out, codons preferred by prokaryotes are selected, a new gene sequence is chemically synthesized, and expressed by genetic engineering technology. The expressed protein can be used for vaccines and rapid detection methods for Staphylococcus aureus The establishment of etc., the present invention relates to the field of genetic engineering technology and diagnostic reagents. Background technique [0002] Staphylococcus aureus is an opportunistic pathogen that can cause disease in humans and animals. It parasitizes human skin and mucosal surfaces as a normal flora and accounts for the majority of nosoco...

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Problems solved by technology

First of all, the samples should be enriched and cultured according to the type of pathogenic bacteria to be detected. The identification results are generally only qualitative but not quantitative, and the time required is long and slow. Generally, it takes 3-4 days to infer the results, and accurate identification takes 5-7 days. sky

Most immunological techniques, such as fluorescently labeled antibody experiments, enzyme-linked immunoassay (ELISA), radioimmunoassay, etc., are widely used, but they require high cost, time-consuming and complicated processing of samples, which limit their use in life. Wide application in

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