All-colloidal silica microcapsule for encapsulating bio-enzyme and preparation method thereof

A biological enzyme and microcapsule technology, which is applied in the field of all-silicon water-core colloidal microcapsules encapsulating biological enzymes and its preparation, can solve the problems of low physical and chemical stability, severe preparation method conditions, and loss of core material encapsulation protection. It can achieve the effects of good mechanical properties and chemical stability, controllable active release, and reusability.

Active Publication Date: 2013-12-25
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The severe conditions of the above preparation method (high temperature, ultraviolet light irradiation) can easily lead to the inactivation of biological enzymes; most of the prepared colloids are organic, organic / inorganic hybrid structures, and their physical and chemical stability are low. It is easy to break during the process, thus losing the encapsulation protection and carrying effect on the core material, and it is not suitable for encapsulation of biological enzymes in organic media for catalysis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A) Ethyl orthosilicate is mixed with methanol at a ratio of 1:10, and ammonia water is added to adjust the pH value of the mixed solution to 12, and stirred at room temperature for 3 hours;

[0036] B) Add modifier C to the solution obtained in the above step A) 10 h 20 -Si(OC 4 h 9 ) 3 , the mass ratio between the amount of modifier added and the organic silicon source is 1:50, and the stirring is continued for 24 hours to obtain a methanol dispersion containing modified nano-silica particles;

[0037] C) The methanol dispersion containing modified nano-silica particles is centrifuged at 5000rpm for 60min at room temperature, and after removing the supernatant, solvent exchange is performed on the precipitated silica: first, the centrifuged precipitate The modified silica nanoparticles were first dispersed in a mixed solution with a volume ratio of methanol and chloroform of 7:3 until no large particle agglomerates were observed with the naked eye, then centrifuged...

Embodiment 2

[0041] A) Ethyl tetrasilicate and ethanol were mixed at a ratio of 1:100, and sodium bicarbonate was added to adjust the pH value of the mixed solution to 8, and stirred at room temperature for 36 hours;

[0042] B) Add modifier C to the solution obtained in the above step A) 5 h 10 -Si(OCH 3 ) 3 , the mass ratio between the modifier addition and the organosilicon source is 1:100, and the stirring is continued for 48 hours to obtain an ethanol dispersion containing modified nano-silica particles;

[0043] C) Centrifuge the ethanol dispersion containing modified nano-silica particles at 7000rpm for 30min at room temperature, remove the supernatant, and perform solvent exchange treatment on the precipitated silica: first, centrifuge the precipitated The modified silica nanoparticles were first dispersed in a mixed solution of ethanol and toluene with a volume ratio of 7:3 until no large particle aggregates were observed with the naked eye, then centrifuged and the supernatant...

Embodiment 3

[0047] A) Methyl orthosilicate is mixed with ethanol at a ratio of 1:50, and ammonia water is added to adjust the pH value of the mixed solution to 11, and stirred at room temperature for 10 h;

[0048] B) Add modifier C18H36-Si(OC2H5)3 to the solution obtained in the above step A), the mass ratio between the amount of modifier added and the organic silicon source is 1:60, and continue stirring for 120h to obtain Ethanol dispersion of silicon oxide particles;

[0049] C) Centrifuge the ethanol dispersion containing modified nano-silica particles at 13,000 rpm for 15 minutes at room temperature, remove the supernatant, and perform solvent exchange treatment on the precipitated silica: first, centrifuge the precipitated The modified silica nanoparticles were first dispersed in a mixed solution of ethanol and tetrahydrofuran with a volume ratio of 7:3 until no large particle agglomerates were observed with the naked eye, then centrifuged and the supernatant was removed; secondly,...

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Abstract

The invention relates to an all-colloidal silica microcapsule for encapsulating bio-enzyme and a preparation method thereof. The preparation method comprises the following steps: firstly, emulsifying a buffer solution containing bio-enzyme and an organic solvent containing modified silicon dioxide nanoparticles to form water / oil emulsion with stable nanoparticles on a two-phase interface; and then, adding silane, and hydrolyzing the silane to adhere and fix the nanoparticles so as to prepare a water-core all-colloidal silica microcapsule for encapsulating bio-enzyme. The preparation conditions of the all-colloidal silica microcapsule provided by the invention are mild, and the stability of activity of the bioenzyme in the organic solvent is realized. The colloidal microcapsule having an all-silicon structure has good physical and chemical stability, so that the dispersion problem of the bioenzyme in an organic medium is solved, and the activity of the bioenzyme in the organic solvent is greatly improved; furthermore, the all-colloidal silica microcapsule has an adjustable and controllable shell wall structure with certain permeability, thereby realizing the controllable activity release and repeated use of the bio-enzyme in the organic medium. The all-colloidal silica microcapsule for encapsulating bioenzyme has a broad application prospect in catalytic reactions of the bio-enzyme in the organic medium.

Description

technical field [0001] The invention relates to an all-silica colloidal microcapsule for encapsulating biological enzymes and a preparation method thereof, in particular to an adjustable shell wall structure formed by bonding one or more layers of silica nanoparticles through silane hydrolysis. All silicon water core colloidal microcapsules encapsulating biological enzymes and a preparation method thereof. Background technique [0002] Colloid body is a novel microcapsule with a semi-permeable shell wall structure, and its shell wall is solidified by the interaction between colloidal particles dispersed in Pickering emulsion oil-water interface. Colloid-encapsulated core materials have received great attention in the fields of medicine, cosmetics, and food, but they are only in their infancy. At present, the colloidal particles that can be used to prepare colloidal microcapsules are polymer microspheres (PS colloidal particles, PMMA colloidal particles, PNiPAAm colloidal pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02
Inventor 阎克路张弛胡春艳
Owner DONGHUA UNIV
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