RGD (Arginine-Glycine-Aspartic Acid) peptide modified double-layer medicine carrying nanometer particle and preparation method thereof
A nanoparticle, RGD sequence technology, applied in the field of biomedicine and nanomedicine, can solve the problems of inability to clinical treatment, difficult to solve the problem of non-selective distribution of drugs in the brain, limited drug amount, etc., to achieve high anti-tumor efficiency and prolong drug effect. Time, effect of reversing drug resistance
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Embodiment 1
[0038] 1. Synthesis of RGD-CS
[0039] Weigh 1 μmol of chitosan (CS) with a molecular weight of 1 μmol, dissolve it in 5 ml of acetic acid, and call it solution A; weigh 16 μmol of RGD (CRGDKGPDC), dissolve it in 2.1 ml of sodium acetate buffer, and call it solution B. Weigh 16 μmol NHS-PEG-MAL and add it to solution A in solid form at room temperature. After vortexing for 30 s, add all the reaction solution to solution B, and continue to react at room temperature for 12 h. Then transfer to an ultrafiltration tube with a molecular weight cut-off of 50kDa, perform ultrafiltration at 4000rpm, 30min 5-6 times, remove unreacted PEG-RGD and RGD, collect the upper liquid and freeze-dry to obtain a white loose solid.
[0040] Or: Weigh 1 μmol chitosan (CS) with a molecular weight of 1 μmol, dissolve it in 5ml acetic acid, and call it solution A; weigh 16 μmol RGD (CRGDKGPDC), dissolve it in 2.1ml sodium acetate buffer, and call it solution B. Weigh 16 μmol of Sulfo-SMCC and add it t...
Embodiment 2
[0044] With the preparation method of Example 1, the CRGDKGPDC in the prescription was changed to CRGDKGPEC with the same molar number, and the dosage of other components in the prescription was fixed to prepare CRGDKGPEC-modified nanoparticles.
Embodiment 3
[0046] With the preparation method of Example 1, the CRGDKGPDC in the prescription was changed to CRGDRGPEC with the same molar number, and the dosage of other components in the prescription was fixed to prepare CRGDRGPEC-modified nanoparticles.
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