Human diploid cell rabies vaccine and preparation method thereof

Abstract

The invention discloses a human diploid cell rabies vaccine and a preparation method thereof, and belongs to the field of vaccines. A CDKHBP-1 strain is inoculated into a human diploid cell WI-38, and the rabies vaccine is obtained through separation and purification. The human diploid cell does not contain any contaminants or oncogenicity, and is obvious superior to an animal cell serving as a medium for producing the vaccine; and the method is suitable for large-scale industrial production, a purification process is perfect, and the vaccine contains a few impurities and has high purity and immunogenicity.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a human diploid cell inactivated vaccine, in particular to a rabies inactivated purified vaccine obtained by inoculating human diploid cells with a rabies virus strain and a preparation method thereof. Background technique [0002] Rabies, rabies, also known as hydrophobia, is an acute viral infectious disease that affects the central nervous system. All warm-blooded animals, including humans, may be infected. It is mostly obtained from the bite of infected animals. Since there is no effective treatment for rabies and the mortality rate is extremely high, vaccination is the only effective means to prevent the occurrence of rabies and ensure people's health. [0003] At present, there are four kinds of rabies vaccines mass-produced in the world: the first kind is the human diploid cell represented by the United States, and the rabies vaccine prepared by concentration and ultracentri...

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Problems solved by technology

[0003] At present, there are four kinds of rabies vaccines mass-produced in the world: the first kind is the human diploid cell represented by the United States, and the rabies vaccine prepared by concentration and ultracentrifugation; the second kind is Vero cell represented by France as the culture substrate, produced Freeze-dried inactivated vaccines, but the tumorigenicity of the cell matrix and the safety of cellular DNA cannot be guaranteed

The third and fourth types are hamster kidneys represented by China and chicken embryos and duck embryos from Japan; these types of vaccine cells are derived from common animals, and there is no guarantee that the cells do not carry exogenous factors, nor can it guarantee the virulence of the cell matrix. Tumor and safety of cellular DNA

[0015] The disadvantage of HDCV is that HDC is not easy to cultivate, and the virus titer of rabies virus cultured on HDC is relatively low, and it can only be cultured in cell flasks with limited space, which makes the vaccine more expensive

In the United States, a course of postexposure treatment with HDCV can cost more than $1,000, limiting the vaccine's use in developing countries