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Method for improving growth rate and yield of oyster mushroom

A technology of growth speed and oyster mushroom, which is applied in the field of agricultural microorganisms, can solve the problems affecting the application, complex methods, and strict requirements for breeding experiment conditions, and achieve the effects of shortening the growth cycle, simple operation process, and increasing growth speed and yield

Inactive Publication Date: 2009-01-28
INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the safety of this transgenic technology has always been controversial, and due to the complexity of the technical method and strict requirements on the conditions of breeding experiments, the application of this method has been affected.

Method used

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  • Method for improving growth rate and yield of oyster mushroom
  • Method for improving growth rate and yield of oyster mushroom
  • Method for improving growth rate and yield of oyster mushroom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for improving the growth rate and output of oyster mushroom, the specific steps are as follows:

[0021] Strain culture:

[0022] Inoculate Pleurotus ostreatus hyphae on standard PDA medium and culture at 25°C for 72 hours; place the cultivated Pleurotus ostreatus dikaryotic mycelia in a 0.10 Tesla magnetic field for 10 minutes; 0.5*0.5cm 2 Strain block, access to special VB 1 -PDA medium test tube, cultivated in the dark at 25°C for 7 days; VB 1 -PDA medium formula: glucose 20g, MgSO 4 1.5g, KH 2 PO 4 3.0g, yeast extract 0.6g, vitamin B 1 0.5mg, agar powder 16g, water 1000ml.

[0023] Fruiting management:

[0024] The mushrooming site can be a large arched shed, a semi-underground mushroom shed, etc. The requirement is that the shed should have good heat preservation, good ventilation, and convenient drainage; clinker should be used for the original species and cultivated species, and the formula is that the ratio of cottonseed hulls, water, and urea ...

Embodiment 2

[0032] The method for improving flat mushroom growth rate and output as described in embodiment 1, difference is:

[0033] In the strain culture step, the Pleurotus ostreatus binucleate hyphae inoculated on standard PDA medium and cultured at 25°C for 72 hours were placed in a magnetic field of 0.15 Tesla for 5 minutes, and the yield was significantly higher than that of ordinary grown flat mushrooms treated with magnetic field magnetization. Mushroom strains, the results are detailed in figure 2 .

Embodiment 3

[0035] The method for improving flat mushroom growth rate and output as described in embodiment 1, difference is:

[0036] In the strain culture step, the Pleurotus ostreatus dikaryotic hyphae inoculated on standard PDA medium and cultured at 25°C for 72 hours were placed in a magnetic field of 0.05 Tesla for 15 minutes, and the yield was significantly higher than that of ordinary grown flat mushrooms treated with magnetic field magnetization. Mushroom strains, the results are detailed in figure 2 .

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Abstract

The invention relates to a method for improving the growth speed and the yield of mushroom and belongs to the technical field of agricultural microbiology; the method comprises the following steps: the mushroom hypha is processed by magnetization by the magnetic field; the magnetizing post culture is carried out; then the existing and skilled technology is adopted to carry out transferring of original seeds, cultivated seeds and produced seeds; and a bag cultivation mode is adopted to complete the fruiting in plastic tents. The method for improving the growth speed and the yield of mushroom of the invention can improve the production trait by controlling the intensity of the magnetic field and the processing time quantitatively.

Description

technical field [0001] The invention relates to a method for increasing the growth rate and output of oyster mushrooms, belonging to the technical field of agricultural microorganisms. Background technique [0002] The improvement of edible fungus strains is generally achieved by domesticating naturally mutated wild species or artificially mutagenizing existing cultivated species. Due to the low frequency of gene mutations caused by the natural environment, there are very few wild mutant varieties, and it takes a lot of time and manpower to improve existing varieties by collecting mutated wild varieties. Artificial mutagenesis can be accomplished by means of physical factors, chemical factors or biotechnology. Among physical factors, neutral particle radiation mainly ultraviolet rays, gamma rays and neutrons are often used. Chemical methods mostly use polyethylene glycol. , Biotechnology includes single spore hybridization, protoplast hybridization and so on. The deficienc...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01G7/04C12N1/14
CPCY02P60/14
Inventor 宫志远李瑾任鹏飞曲玲任海霞姚强
Owner INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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