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Albizzia plant extraction composition for inhibiting angiogenesis and preparation and application thereof

A plant extract, angiogenesis technology, applied in the field of traditional Chinese medicine, can solve the problem of unclear anti-cancer mechanism, and achieve the effects of less drug resistance, inhibition of angiogenesis, and high curative effect

Active Publication Date: 2008-04-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Albizia Julibrissin extract has a wide range of in vivo and in vitro anti-tumor cell proliferation activities, but its anti-cancer mechanism is still unclear, which limits its clinical application of anti-cancer effects 【15, Tsuyoshi Ikeda, Tetsuya Kajimoto, Toshihiro Nohara, Jun -eiKino, Chi-Huey Wong.Preparation of a neoglycolipid carrying the oligosaccharidecomponent of saponin from Albizzia julibrissin.Tetrahedron Letters.1995,36(9):1509-1510.16, Kun Zou, Yuying Zhao, Guangzhong Tu, Jingrong Cui, Zhonghua Jia and Ruyi Zhang.Two diastereomeric saponins with cytotoxic activity from Albiziajulibrissin.Carbohydrate Research.2000, 324(3):182-188.17, Kun Zou, Yuying Zhao, Guangzhong Tu, Junhua Zheng, Rulibris Zhang.A New IsomerJ ofJulibroside J2 from Albizia Asian Natural Products Research.1998, 1(1): 59-66.18, Zou Kun, Zhao Yuying, Li Deyu, Xu Feng, Zheng Junhua, Zhang Ruyi. Fat-soluble components of Albizia Julibrissin. Journal of Beijing Medical University, 1999, 31(1): 32-34.]

Method used

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  • Albizzia plant extraction composition for inhibiting angiogenesis and preparation and application thereof
  • Albizzia plant extraction composition for inhibiting angiogenesis and preparation and application thereof
  • Albizzia plant extraction composition for inhibiting angiogenesis and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: The inhibitory effect of Albizia plant extract on the proliferation of human vascular endothelial cells.

[0028] Human vascular endothelial cells (HMEC, gift from the French National Institute of Health and Medicine) were placed in MCDB- containing 1mmol glutamine, 1μg / mL hydrocortisone, 10ng / mL epidermal growth factor (EGF) and 15% calf serum Cultivate in 131 medium (37℃, 5% CO 2 , 95% humidity). Take the logarithmic growth phase cells and take 10 5 cell / mL cells are seeded in 96-well plates, 100μL / well, each group has 4 parallel wells, placed at 37℃, 5% humidified CO 2 In the incubator overnight. Add different concentrations (10-100μg / mL) of Albizia plant extracts, use water as a negative control, after 48 hours of culture, remove the supernatant, gently add 100μL of 10% trichloroacetic acid to each well for fixation, and let stand for 5 minutes Move to 4°C for 1 hour, discard the fixative, wash 5 times with deionized water, and air dry. Add 100 μL of 0.4% sul...

Embodiment 2

[0031] Example 2: The inhibitory effect of Albizia plant extract on the angiogenesis ability.

[0032] 2.1 Albizia plant extract inhibits angiogenesis of chicken embryo allantoic membrane (CAM experiment)

[0033] White skin breeding eggs, refer to Zhang Shucheng's method [19, Zhang Shucheng, Wu Zhikui, Wang Lei, etc.. Application of chicken embryo chorioallantoic membrane test model to study the angiogenic activity and effect of traditional Chinese medicine. Chinese Journal of Basic Medicine in Traditional Chinese Medicine, 1999, 5: 16- 19.] Preparation of chicken embryo allantoic membrane. A microporous filter membrane disc with a diameter of 6 mm was prepared with a hole punch, and sterilized by high pressure with humid heat. Set up the Albizia plant extract administration group (10μL, 1g crude drug / mL) and the physiological saline negative control group. Drop the medicine on the filter paper to make a medicine film, which is dried for later use. The chicken embryos were cultur...

Embodiment 3

[0041] Example 3: Lewis lung cancer metastasis model mice analysis of the inhibitory effect of Albizia plant extracts on tumors in vivo

[0042] Fifteen male inbred C57BL / 6 mice weighing 14-18g were selected, and the prepared Lewis lung cancer cell suspension was inoculated into the right axillary skin of the mouse at 0.2 mL / mouse (about 1×10 viable cells). 6 ). Randomly divided into 3 groups, 5 in each group: normal saline control group, licorice extract negative control group (0.4mL, 1g crude drug / mL), Albizia plant extract medication group (0.4mL, 1g crude drug / mL). Four days after inoculation with Lewis lung cancer cells, the rats were given intragastrically once a day for a total of 4 weeks of treatment. On the 28th day, all mice were sacrificed by cervical dislocation, the tumors were removed intact, and part of the tumor tissues were taken, conventional paraffin sections or frozen sections, HE stained, and observed under light microscope (×100).

[0043] The results are sho...

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Abstract

The invention discloses a composition of albiziz durazz extract for inhibiting angiogenesis and preparation and application, belonging to the field of tradition Chinese medicine applied technique. The invention relates to a composition containing albiziz durazz extract, process for preparation and new usage. The invention discovers for the first time that the composition containing albiziz durazz extract can inhibit angiogenesis activity, and thereby the invention can be used for curing and preventing diseases relating to angiogenesis and also can be used for treatment of tumor chemotherapy and / or auxiliary chemotherapy. The composition of albiziz durazz extract of the invention contains one or a plurality of anti-angiogenesis active components. The invention skillfully takes angiogenesis as the target for curing diseases, studies and expounds medicinal curative effect and mechanism of composition containing albiziz durazz extract. The composition containing albiziz durazz extract can be used as drugs, health care products and / or cosmetics and the like.

Description

Technical field [0001] The composition of Albizia plant extracts for inhibiting angiogenesis and its preparation and application belong to the technical field of traditional Chinese medicine application. The present invention relates to an extract prepared from Albizia plants as a raw material, which has the activity of inhibiting angiogenesis, and clarifies its preparation method and new uses in medicine, food and cosmetics. Background technique [0002] In the human body, as long as there is organ formation, there will be the formation of new blood vessels, or new formation of blood vessels, which is necessary for the transportation of oxygen, nutrients, and metabolites. Blood vessel formation only occurs in the early embryonic stage. These types of blood vessel formation are often regulated by certain factors and are intermittent. The doubling time of vascular endothelial cells in a normal human body is one year. On the other hand, continuous and unregulated angiogenesis occur...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61K9/06A61K9/08A61K9/10A61K9/107A61K9/14A61K9/16A61K9/20A61K9/48A61P35/00A61P29/00A61P27/06A61P27/02A61P19/02A61P17/00A61P17/06A61P17/10A61P9/10A61K125/00A61K127/00A61K129/00A61K131/00A61K133/00
Inventor 金坚花慧张莲芬冯磊陆核李红
Owner JIANGNAN UNIV
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