The invention relates to a
cutting type in-situ fixing device of deep-sea mussels and other large organisms based on ROV, and a fixing method thereof. A fixed cabin and a driving oil cylinder are respectively installed on the ROV, the ROV provides hydraulic oil for the driving oil cylinder, one end of the fixed cabin is hermetically connected with a
front cover, the other end of the fixed cabin ishermetically connected with a storage cabin, the other end of the storage cabin is hermetically connected with a back cover, a knife rest with a
cutting knife is installed in the fixed cabin, and a delivery port is formed in the storage cabin; a
rigid body is inserted into the storage cabin after passing through the back cover and is connected with one side of a
piston, the other side of the
piston is connected with a
cutting knife pressing plate, and the
piston is hermetically and slidably connected with the inner wall of the storage cabin; and a fixed solution is stored in a fixed solutionstorage bag, and the fixed solution storage bag communicates with the interior of the fixed cabin through a rubber tube passage. The cutting type in-situ fixing device provided by the invention meetsthe in-situ fixing needs of the mussels and other large organisms of a deep-sea
ecosystem, is scarcely influenced by the sampling depth, high in
corrosion resistance, large in storage volume, and flexible and stable to work, and can quickly and effectively fix large biological samples.