The invention provides a manufacturing method of a propolis comb foundation. The method comprises the following steps that A, a propolis extract is placed in a refrigerator to be frozen at the temperature of -5--20 DEG C for 24 h, taken out and smashed to be prepared into propolis powder with 50-100 meshes, and the propolis powder is stored at the low temperature for use; B, a substrate is heated and molten, the propolis powder is added into the molten substrate, stirring is carried out to enable the propolis powder and the molten substrate to be mixed to be uniform, and propolis mixed wax liquid is prepared, wherein the adding amount of the propolis powder is 0.2-2% of the weight of the substrate; C, the propolis mixed wax liquid is cooled, tabletted, loaded to a propolis comb machine, drawn, pressed and cut to be propolis comb foundation tablets. The manufacturing method better accords with the natural biological characteristics of bees, can be widely applied to preventing and treating various bee diseases, and has the advantages that production is easy and convenient, the cost is low, use is safe, and environmental protection is achieved; meanwhile, propolis and beewax are both obtained from a swarm and used for the swarm, and can be completely used for comb honey production, which is unparalleled.